KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
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additional information | - |
additional information | Km-values fpr 14-mers and various substituted duplexes | Escherichia coli |
Organism | UniProt | Comment | Textmining |
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Escherichia coli | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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S-adenosyl-L-methionine + DNA adenine | canonical 14mers and various substituted duplexes. Non-selfcomplementary tetradecamer duplex d[GCCGGATCTAGACG]*d[CGTCTAGATCCGGC] containing the hemimethylated GATC target sequence on one or the other strand and modifications in the GATC target sequence of the complementary strands. Large differences in DNA methylation of duplexes containing single dI or dG substitutions of the Dam recognition site are observed compared with the canonical substrate, if the substitution involves the top strand, on the G-C rich side. Substitution in either strand by uracil or 5-ethyluracil result in small perturbation of the methylation patterns. When 2,6-diamino-purine replaces the adenine to be methylated, small but significant methylation is observed | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
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