Cloned (Comment) | Organism |
---|---|
subcloned into pET21b overexpression in Escherichia coli | Caulobacter vibrioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Caulobacter vibrioides | the enzyme is involved in cell cycle regulation of Caulobacter crescentus | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter vibrioides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Caulobacter vibrioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | the enzyme preferentially binds DNA before S-adenosylmethionine. S-Adenosylhomocysteine is preferentially released from the enzyme before fully methylated DNA. Binding of both substrates and methylation occurs first in a rapid step followed by regeneration of enzyme in a second rate-limiting step | Caulobacter vibrioides | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | the enzyme is involved in cell cycle regulation of Caulobacter crescentus | Caulobacter vibrioides | S-adenosyl-L-homocysteine + DNA 6-methylaminopurine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
fully active | Caulobacter vibrioides |
30 | - |
thermally inactivated within 20 min | Caulobacter vibrioides |