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Literature summary for 2.1.1.71 extracted from

  • Morita, S.Y.; Takeuchi, A.; Kitagawa, S.
    Functional analysis of two isoforms of phosphatidylethanolamine N-methyltransferase (2010), Biochem. J., 432, 387-398.
    View publication on PubMed
Search for more literature for 2.1.1.71

Cloned(Commentary)

Cloned (Comment) Organism
stably expressed in human embryonic kidney-293 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 SDS-PAGE, PEMT-S Homo sapiens
26000
-
 SDS-PAGE, PEMT-L Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylated with high-mannose oligosaccharides Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity of PEMT-S is much higher than of its isoform PEMT-L Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
-
 Homo sapiens S-adenosyl-L-homocysteine + phosphatidyl-N,N-dimethylethanolamine
-
 ?

Synonyms

Synonyms Comment Organism
PEMT-L longer isoform of PEMT Homo sapiens
PEMT-S shorter isoform of PEMT Homo sapiens
phosphatidylethanolamine N-methyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens the expression level of PEMT-L is 4.4fold higher than that of PEMT-S down
Homo sapiens the expression level of PEMT-L is 4.4fold higher than that of PEMT-S up

General Information

General Information Comment Organism
metabolism PEMT-L and PEMT-S expression increase the cellular phosphatidylcholine content, whereas the phosphatidylethanolamine content is decreased by PEMT-S expression, but is hardly affected by PEMT-L expression. The cellular content of phosphatidylserine is also reduced by the expression of PEMT-L or PEMT-S Homo sapiens
physiological function PEMT-L and PEMT-S expression promote the proliferation of HEK-293 cells Homo sapiens