Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.70 extracted from

  • Hauffe, K.D.; Hahlbrock, K.; Scheel, D.
    Elicitor-stimulated furanocoumarin biosynthesis in cultured parsley cells: S-adenosyl-L-methionine:bergaptol and S-adenosyl-L-methionine:xanthotoxol O-methyltransferase (1986), Z. Naturforsch. C, 41, 228-239.
No PubMed abstract available
Search for more literature for 2.1.1.70

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification and storage Petroselinum crispum

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
 Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
 S-adenosyl-L-methionine
-
 Petroselinum crispum
0.0098
-
 xanthotoxol
-
 Petroselinum crispum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Petroselinum crispum
67000
-
 gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + xanthotoxol Petroselinum crispum final methylation in the biosynthesis of the furanocoumarin xanthotoxin S-adenosyl-L-homocysteine + xanthotoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
 cv Hamburger Schnitt
-

Purification (Commentary)

Purification (Comment) Organism
-
 Petroselinum crispum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture from leaf petiole, cells treated with an elicitor from Phytophtora megasperma, no activity in untreated cells Petroselinum crispum
-
petiole cell suspension culture Petroselinum crispum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.155
-
-
 Petroselinum crispum

Storage Stability

Storage Stability Organism
-20°C, stable for 20 days in 50% v/v glycerol Petroselinum crispum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 5-hydroxyxanthotoxin 0.7% of the activity with xanthotoxol Petroselinum crispum S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
S-adenosyl-L-methionine + 8-hydroxybergapten 2% of the activity with xanthotoxol Petroselinum crispum S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
S-adenosyl-L-methionine + bergaptol 0.5% of the activity with xanthotoxol Petroselinum crispum S-adenosyl-L-homocysteine + bergapten
-
 ?
S-adenosyl-L-methionine + daphnetin 0.6% of the activity with xanthotoxol Petroselinum crispum S-adenosyl-L-homocysteine + hydrangetin
-
 ?
S-adenosyl-L-methionine + xanthotoxol
-
 Petroselinum crispum S-adenosyl-L-homocysteine + xanthotoxin
-
 ?
S-adenosyl-L-methionine + xanthotoxol final methylation in the biosynthesis of the furanocoumarin xanthotoxin Petroselinum crispum S-adenosyl-L-homocysteine + xanthotoxin
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Petroselinum crispum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.5 8
-
 Petroselinum crispum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 30 min, inactivation below Petroselinum crispum