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Literature summary for 2.1.1.68 extracted from

  • Byeon, Y.; Choi, G.H.; Lee, H.Y.; Back, K.
    Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice (2015), J. Exp. Bot., 66, 6917-6925 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.68

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
caffeic acid 23% residual activity at 0.1 mM Oryza sativa
quercetin 23% residual activity at 0.005 mM Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.243
-
 N-acetylserotonin at pH 7.8 and 37°C Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Oryza sativa 5737
-

Organism

Organism UniProt Comment Textmining
Oryza sativa Q6ZD89
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + N-acetylserotonin
-
 Oryza sativa S-adenosyl-L-homocysteine + melatonin
-
 ?

Synonyms

Synonyms Comment Organism
caffeic acid O-methyltransferase
-
 Oryza sativa
COMT
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Oryza sativa

General Information

General Information Comment Organism
metabolism transgenic rice plants overexpressing the enzyme show an increase in melatonin levels Oryza sativa