Cloned (Comment) | Organism |
---|---|
cloning from phage library, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), subcloning in strain JM109 | Vanilla planifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
5-hydroxyferulic acid ethyl ester | pH and temperature not specified in the publication | Vanilla planifolia | |
0.023 | - |
caffeic acid ethyl ester | pH and temperature not specified in the publication | Vanilla planifolia | |
0.032 | - |
caffeoyl aldehyde | pH and temperature not specified in the publication | Vanilla planifolia | |
0.041 | - |
5-hydroxyconiferaldehyde | pH and temperature not specified in the publication | Vanilla planifolia | |
0.091 | - |
5-hydroxyferulic acid | pH and temperature not specified in the publication | Vanilla planifolia | |
0.25 | - |
caffeic acid | pH and temperature not specified in the publication | Vanilla planifolia | |
0.255 | - |
3,4-Dihydroxybenzaldehyde | pH and temperature not specified in the publication | Vanilla planifolia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40659 | - |
x * 42000, SDS-PAGE, x * 40659, sequence calculation | Vanilla planifolia |
42000 | - |
x * 42000, SDS-PAGE, x * 40659, sequence calculation | Vanilla planifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde | Vanilla planifolia | 3,4-dihydroxybenzaldehyde is not the preferred substrate | S-adenosyl-L-homocysteine + vanillin | i.e. 4-hydroxy-3-methoxybenzaldehyde | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vanilla planifolia | Q6Q796 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially by adenosine-agarose affinity chromatography, 18.2fold for 3,4-dihydroxybenzaldehyde-O-methyltransferase activity and 16.7fold for caffeic acid O-methyltransferase activity | Vanilla planifolia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | low expression level | Vanilla planifolia | - |
additional information | tissue distribution by immunohistochemic analysis | Vanilla planifolia | - |
pod | low expression level | Vanilla planifolia | - |
root | strong expression level | Vanilla planifolia | - |
stem | strong expression level | Vanilla planifolia | - |
tissue culture | strong expression level | Vanilla planifolia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, overview | Vanilla planifolia | ? | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde | 3,4-dihydroxybenzaldehyde is not the preferred substrate | Vanilla planifolia | S-adenosyl-L-homocysteine + vanillin | i.e. 4-hydroxy-3-methoxybenzaldehyde | ? | |
S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde | 3,4-dihydroxy-benzaldehyde is not the preferred substrate | Vanilla planifolia | S-adenosyl-L-homocysteine + vanillin | i.e. 4-hydroxy-3-methoxybenzaldehyde | ? | |
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Vanilla planifolia | S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | - |
Vanilla planifolia | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid ethyl ester | - |
Vanilla planifolia | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + caffeic acid | - |
Vanilla planifolia | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + caffeic acid ethyl ester | - |
Vanilla planifolia | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl aldehyde | - |
Vanilla planifolia | S-adenosyl-L-homocysteine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE, x * 40659, sequence calculation | Vanilla planifolia |
Synonyms | Comment | Organism |
---|---|---|
3,4-dihydroxybenzaldehyde-O-methyltransferase | - |
Vanilla planifolia |
caffeic acid O-methyltransferase | - |
Vanilla planifolia |
COMT | - |
Vanilla planifolia |
DOMT | - |
Vanilla planifolia |
O-methytransferase | - |
Vanilla planifolia |
OMT | - |
Vanilla planifolia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Vanilla planifolia |
General Information | Comment | Organism |
---|---|---|
metabolism | 3,4-dihydroxybenzaldehyde is not the preferred substrate. The higher catalytic efficiency of the purified recombinant enzyme with the substrates caffeoyl aldehyde and 5-hydroxyconiferaldehyde, and its tissue distribution, suggest this methyltransferase may primarily unction in lignin biosynthesis | Vanilla planifolia |