Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Medicago sativa |
Crystallization (Comment) | Organism |
---|---|
crystallization from polyethylene glycol solution, 2.2 A resolution, complex with S-adenosyl-L-homocysteine and ferulic acid | Medicago sativa |
crystallization from polyethylene glycol solution, 2.4 A resolution, complex with S-adenosyl-L-homocysteine and 5-hydroxyconiferaldehyde | Medicago sativa |
Protein Variants | Comment | Organism |
---|---|---|
A162T | same reactivity as wild type | Medicago sativa |
F172Y | no reaction with caffeic acid, with other substrates same reactivity as wild type | Medicago sativa |
H183K | no reaction with caffeic acid, with other substrates same reactivity as wild type | Medicago sativa |
L136Y | same reactivity as wild type | Medicago sativa |
M130L | no reaction with caffeic acid and 5-hydroxyferulic acid, with other substrates same reactivity as wild type | Medicago sativa |
N131K | same reactivity as wild type | Medicago sativa |
N324Y | no reaction with caffeic acid, with other substrates same reactivity as wild type | Medicago sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
5-hydroxyconiferaldehyde | wild type | Medicago sativa | |
0.0075 | - |
5-hydroxyconiferyl alcohol | wild type | Medicago sativa | |
0.01 | - |
5-hydroxyferulic acid | wild type | Medicago sativa | |
0.01 | - |
caffeoyl aldehyde | wild type | Medicago sativa | |
0.013 | - |
caffeoyl alcohol | wild type | Medicago sativa | |
0.043 | - |
caffeic acid | wild type | Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | Medicago sativa | i.e. caffeic acid, involved in the biosynthesis of lignin | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | P28002 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | - |
Medicago sativa | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate | i.e. caffeic acid, involved in the biosynthesis of lignin | Medicago sativa | S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Medicago sativa | S-adenosyl-L-homocysteine + sinapaldehyde | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol | - |
Medicago sativa | S-adenosyl-L-homocysteine + 5-hydroxy-3-methoxyconiferyl alcohol | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | - |
Medicago sativa | S-adenosyl-L-homocysteine + sinapic acid | - |
? | |
S-adenosyl-L-methionine + caffeoyl alcohol | - |
Medicago sativa | S-adenosyl-L-homocysteine + 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol | - |
? | |
S-adenosyl-L-methionine + caffeoyl aldehyde | - |
Medicago sativa | S-adenosyl-L-homocysteine + coniferyl aldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
COMT | - |
Medicago sativa |