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Literature summary for 2.1.1.67 extracted from

  • Wennerstrand, P.; Blissing, A.; Martensson, L.G.
    In vitro protein stability of two naturally occurring thiopurine S-methyltransferase variants biophysical characterization of TPMT*6 and TPMT*8 (2017), ACS Omega, 2, 4991-4999.
    View publication on PubMed
Search for more literature for 2.1.1.67

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
R215H the mutant shows intermediate in vivo activity compared to the wild type enzyme Homo sapiens
Y180F the mutant shows low in vivo activity compared to the wild type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P51580
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 6-mercaptopurine
-
 Homo sapiens S-adenosyl-L-homocysteine + 6-methylmercaptopurine
-
 ?

Synonyms

Synonyms Comment Organism
TPMT
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46.4
-
 melting temperature of the wild type enzyme without ligand Homo sapiens
46.8
-
 melting temperature of the wild type enzyme in complex with S-adenosyl-L-methionine Homo sapiens