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Literature summary for 2.1.1.67 extracted from
- Structural characteristics determine the cause of the low enzyme activity of two thiopurine S-methyltransferase allelic variants: a biophysical characterization of TPMT*2 and TPMT*5 (2012), Biochemistry, 51, 5912-5920.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A80P | the mutant is substantially destabilized and shows 47% of wild type activity | Homo sapiens |
| L49S | the mutant shows much greater stability comparable to that of wild type enzyme and exhibits 14% of wild type activity | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + 6-mercaptopurine | - |
Homo sapiens | S-adenosyl-L-homocysteine + 6-methylmercaptopurine | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPMT | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
the melting temperature of the wild type enzyme is at 55°C | Homo sapiens |
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