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Literature summary for 2.1.1.63 extracted from

  • Gronemeyer, T.; Chidley, C.; Juillerat, A.; Heinis, C.; Johnsson, K.
    Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling (2006), Protein Eng. Des. Sel., 19, 309-316.
    View publication on PubMed
Search for more literature for 2.1.1.63

Cloned(Commentary)

Cloned (Comment) Organism
ligated into the pHybLex/Zeo plasmid and electroporated into Escherichia coli XL1-Blue, yielding a library of at least 320000 independent clones, genes of selected AGT mutants subcloned into the pGEX-2T plasmid, allowing for the expression of the AGT mutants as GST fusion proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
A154T ca. 4fold increased activity, AGT mutant selected using phage display Homo sapiens
A41D/S115T/I151N 1.6fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
D42E/A51T/A64V/K104M 0.5fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
D42E/P47L/V155L/K178M 1.9fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
E110D/L120M 0.7fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
E25K 0.7fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
E92D/I151V/R175W 1.0fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
F79I/V88I/F89L 0.7fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
G122C 0.9fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
H71Y/A154T 3.3fold increased activity, AGT mutant selected using phage display Homo sapiens
K104E/T127A/A154T 4.5fold increased activity, AGT mutant selected using phage display Homo sapiens
K107R/A154T 4.7fold increased activity, AGT mutant selected using phage display Homo sapiens
K8R/K104E/I151T 1.4fold increased activity, AGT mutant selected using phage display Homo sapiens
K8T/A51T/I112V/A154T 5.5fold increased activity, AGT mutant selected using phage display Homo sapiens
K8T/T127A/A154T/H174R 5.8fold increased activity, AGT mutant selected using phage display Homo sapiens
L33F/A68T 2.1fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
L33F/N123Y 2.2fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
L33F/V44A/V52A/A154T 6.1fold increased activity, AGT mutant selected using phage display Homo sapiens
L66M/K131R 1.5fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
M1V/V164M 1.9fold increased activity, AGT mutant selected using phage display Homo sapiens
additional information MAGT decreased activity can be restored by combination of saturation mutagenesis of residues 150-154 and 31-35 from phage display and yeast three-hybrid system selections, reveals a mutant with 17fold higher activity than MAGT and a 52fold higher activity than wild-type AGT, is the most active AGT mutant against O6-benzylguanine derivatives described so far Homo sapiens
N123S 1.3fold increased activity, AGT mutant selected using phage display Homo sapiens
N150D 1.8fold increased activity, AGT mutant selected using phage display Homo sapiens
N157/S159/C62A/C150N/G131K/G132T/M134L/R135S/Q115S/Q116H/K125A/A127T/R128A/S151I/S152N called MAGT with 15 different mutations in a single protein, has23fold increase in activity relative to wild-type, is resistant against N9-substituted BG derivatives used for inhibition of wild-type, shows suppressed affinity towards DNA Homo sapiens
Q90R/K101N/F108I/V164L 1.2fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
R175L 0.6fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
T11I/N67K/Q72L 1.0fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
T127A 2.2fold increased activity, AGT mutant selected using phage display Homo sapiens
T38M/A41D/A64T/G173C 0.8fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
V149I/A154T 4.5fold increased activity, AGT mutant selected using phage display Homo sapiens
V44G/V106A/I151T/A170T 1.4fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens
V46A/A50V/P58V/A154T 3.4fold increased activity, AGT mutant selected using phage display Homo sapiens
V52A/I151S/K178E 2.3fold increased activity, AGT mutant selected using phage display Homo sapiens
V52I/V164M 2.1fold increased activity, AGT mutant selected using yeast three-hybrid system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
O6-benzylguanine
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Synonyms

Synonyms Comment Organism
AGT
-
 Homo sapiens
O6-alkylguanine-DNA alkyltransferase
-
 Homo sapiens