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Literature summary for 2.1.1.63 extracted from

  • Juillerat, A.; Heinis, C.; Sielaff, I.; Barnikow, J.; Jaccard, H.; Kunz, B.; Terskikh, A.; Johnsson, K.
    Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells (2005), Chembiochem, 6, 1263-1269.
    View publication on PubMed
Search for more literature for 2.1.1.63

Application

Application Comment Organism
analysis ability to specifically label AGT fusion proteins in the presence of endogenous AGT, after brief incubation of the cells with a small-molecule inhibitor, may significantly broaden the scope of application of AGT fusion proteins for studying protein function in living cells Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
mutants subcloned into pGEX-2T for expression as a GST–AGT fusion, or into pET15b for expression as a 6 x His–AGT fusion protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information AGT mutants resistant to inactivation by N9-cyclopentyl-O6-(4-bromothenyl)guanine Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N9-cyclopentyl-O6-(4-bromothenyl)guanine efficient inhibitor of wild-type AGT Homo sapiens
O6-(4-bromothenyl)guanine
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P16455
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
AGT
-
 Homo sapiens
O6-alkylguanine-DNA alkyltransferase
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0005
-
-
 Homo sapiens N9-cyclopentyl-O6-(4-bromothenyl)guanine