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Literature summary for 2.1.1.63 extracted from

  • Fang, Q.; Kanugula, S.; Pegg, A.E.
    Function of domains of human O6-alkylguanine-DNA alkyltransferase (2005), Biochemistry, 44, 15396-15405.
    View publication on PubMed
Search for more literature for 2.1.1.63

Application

Application Comment Organism
analysis N-terminal domain plays a critical structural role in maintaining an active configuration of the C-terminal domain, N-hAGT and C-hAGT domains can protect from N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli GWR-109 cells Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-hAGT and C-hAGT in Escherichia coli XL1-Blue cells. N-hAGT, C-hAGT and full length AGT expressed from the pQE-30 vector in Escherichia coli GWR-109 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C24A mutation of Cys24 prevents the zinc-dependent alkyl transferase by N-terminal domain human AGT Homo sapiens
C5A mutation of Cys5 prevents the zinc-dependent alkyl transferase by N-terminal domain human AGT Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
O6-benzylguanine
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ N-terminal domain contains a bound zinc atom, and zinc binding confers a mechanistic enhancement to repair activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by immobilized metal affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing 6-O-methylguanine + [protein]-L-cysteine C-terminal domain is totally inactive, the N-terminal domain has a very weak but definite activity that is totally dependent on the refolding being carried out in the presence of zinc ions Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
AGT
-
 Homo sapiens
C-hAGT
-
 Homo sapiens
N-hAGT
-
 Homo sapiens
O6-alkylguanine-DNA alkyltransferase
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00009
-
 N-hAGT and C-hAGT complex Homo sapiens O6-benzylguanine
0.00022
-
 N-hAGT Homo sapiens O6-benzylguanine