Literature summary for 2.1.1.59 extracted from

DiMaria, P.; Polastro, E.; DeLange, R.J.; Kim, S.; Paik, W.K.
Studies on cytochrome c methylation in yeast (1979), J. Biol. Chem., 254, 4645-4652.

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Inhibitors

Inhibitors	Comment	Organism	Structure
S-adenosyl-L-homocysteine	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	S-adenosyl-L-methionine	-	Saccharomyces cerevisiae
1.33	-	[cytochrome c]-L-lysine	of horse heart	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
97000	-	gel filtration	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + cytochrome c L-lysine	Saccharomyces cerevisiae	in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	-	?
S-adenosyl-L-methionine + cytochrome c L-lysine	Saccharomyces cerevisiae	regulation of enzyme and cytochrome c substrate is not in parallel manner	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partially	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000117	-	partially purified enzyme	Saccharomyces cerevisiae

Storage Stability

Storage Stability	Organism
-20°C, pH 7.0, crude extract supernatant, stable	Saccharomyces cerevisiae
purified enzyme is stabilized by addition of 66% ammonium sulfate during storage	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + cytochrome c L-lysine	specific for unmethylated cytochrome c	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	a single lysine residue at position 72 of horse heart cytochrome c is methylated	?
S-adenosyl-L-methionine + cytochrome c L-lysine	specific for unmethylated cytochrome c	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	9:17:74	?
S-adenosyl-L-methionine + cytochrome c L-lysine	specific for unmethylated cytochrome c	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4	?
S-adenosyl-L-methionine + cytochrome c L-lysine	horse heart cytochrome c-72	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	a single lysine residue at position 72 of horse heart cytochrome c is methylated	?
S-adenosyl-L-methionine + cytochrome c L-lysine	horse heart cytochrome c-72	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	9:17:74	?
S-adenosyl-L-methionine + cytochrome c L-lysine	horse heart cytochrome c-72	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4	?
S-adenosyl-L-methionine + cytochrome c L-lysine	overview: substrate activity of various cytochromes c	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	a single lysine residue at position 72 of horse heart cytochrome c is methylated	?
S-adenosyl-L-methionine + cytochrome c L-lysine	overview: substrate activity of various cytochromes c	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	9:17:74	?
S-adenosyl-L-methionine + cytochrome c L-lysine	overview: substrate activity of various cytochromes c	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4	?
S-adenosyl-L-methionine + cytochrome c L-lysine	in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	-	?
S-adenosyl-L-methionine + cytochrome c L-lysine	regulation of enzyme and cytochrome c substrate is not in parallel manner	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine	-	?
S-adenosyl-L-methionine + [cytochrome c]-L-lysine	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine	-	r

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)