Cloned (Comment) | Organism |
---|---|
baculovirus expression system | bluetongue virus |
Crystallization (Comment) | Organism |
---|---|
to 2.5 A resolution. Residues 175 to 377 encode a distinct 2'-O MT domain segregated from the other domains and responsible for methylation of cap0 structure | bluetongue virus |
Protein Variants | Comment | Organism |
---|---|---|
D265E | complete loss of 2'-O-methylation activity, Bluetongue virus replication in cells is abrogated | bluetongue virus |
D265V | complete loss of 2'-O-methylation activity, Bluetongue virus replication in cells is abrogated | bluetongue virus |
N311A | 2fold reduction of 2'-O-methylation activity, Bluetongue virus replication in cells is abrogated | bluetongue virus |
N311A/Y334/R367A | 2fold reduction of 2'-O-methylation activity, Bluetongue virus replication in cells is abrogated | bluetongue virus |
R367A | mutation does not affect methyl group transfer | bluetongue virus |
Y334A | mutation does not affect methyl group transfer | bluetongue virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
x * 74000, calculated | bluetongue virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
bluetongue virus | C5IWW0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7Me-GpppG | - |
bluetongue virus | S-adenosyl-L-homocysteine + 7Me-Gppp-2'OMe-G | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 74000, calculated | bluetongue virus |
Synonyms | Comment | Organism |
---|---|---|
VP4 | - |
bluetongue virus |