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Literature summary for 2.1.1.57 extracted from

  • Chung, K.Y.; Dong, H.; Chao, A.T.; Shi, P.Y.; Lescar, J.; Lim, S.P.
    Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2-O methyltransferase activity in dengue virus (2010), Virology, 402, 52-60.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Dengue virus

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-homocysteine
-
Dengue virus
sinefungin
-
Dengue virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
S-adenosyl-L-methionine apparent value, in glycine buffer (pH 10.0), at 22°C Dengue virus
1.8
-
m7G(5')pppG-RNA apparent value, in glycine buffer (pH 10.0), at 22°C Dengue virus

Organism

Organism UniProt Comment Textmining
Dengue virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GSTPrep 16/10 column chromatography Dengue virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + m7G(5')pppG-RNA m7GTP capped nucleotides 1-110. 2'-O MTase activity is seen only in glycine buffer, at pH 10, with a minimum RNA length of 20 nucleotides needed Dengue virus S-adenosyl-L-homocysteine + m7G(5')pppGm-RNA
-
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Synonyms

Synonyms Comment Organism
2'-O MTase
-
Dengue virus
2'O methyltransferase
-
Dengue virus
NS5 MTase
-
Dengue virus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000041
-
in glycine buffer (pH 10.0), at 22°C Dengue virus sinefungin
0.00049
-
in glycine buffer (pH 10.0), at 22°C Dengue virus S-adenosyl-L-homocysteine