BRENDA - Enzyme Database
show all sequences of 2.1.1.57

Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2-O methyltransferase activity in dengue virus

Chung, K.Y.; Dong, H.; Chao, A.T.; Shi, P.Y.; Lescar, J.; Lim, S.P.; Virology 402, 52-60 (2010)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21 cells
Dengue virus
Inhibitors
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
-
Dengue virus
sinefungin
-
Dengue virus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
S-adenosyl-L-methionine
apparent value, in glycine buffer (pH 10.0), at 22C
Dengue virus
1.8
-
m7G(5')pppG-RNA
apparent value, in glycine buffer (pH 10.0), at 22C
Dengue virus
Organism
Organism
UniProt
Commentary
Textmining
Dengue virus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
GSTPrep 16/10 column chromatography
Dengue virus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + m7G(5')pppG-RNA
m7GTP capped nucleotides 1-110. 2'-O MTase activity is seen only in glycine buffer, at pH 10, with a minimum RNA length of 20 nucleotides needed
721099
Dengue virus
S-adenosyl-L-homocysteine + m7G(5')pppGm-RNA
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
2'-O MTase
-
Dengue virus
2'O methyltransferase
-
Dengue virus
NS5 MTase
-
Dengue virus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000041
-
in glycine buffer (pH 10.0), at 22C
Dengue virus
sinefungin
0.00049
-
in glycine buffer (pH 10.0), at 22C
Dengue virus
S-adenosyl-L-homocysteine
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Dengue virus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000041
-
in glycine buffer (pH 10.0), at 22C
Dengue virus
sinefungin
0.00049
-
in glycine buffer (pH 10.0), at 22C
Dengue virus
S-adenosyl-L-homocysteine
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
S-adenosyl-L-homocysteine
-
Dengue virus
sinefungin
-
Dengue virus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.19
-
S-adenosyl-L-methionine
apparent value, in glycine buffer (pH 10.0), at 22C
Dengue virus
1.8
-
m7G(5')pppG-RNA
apparent value, in glycine buffer (pH 10.0), at 22C
Dengue virus
Purification (Commentary) (protein specific)
Commentary
Organism
GSTPrep 16/10 column chromatography
Dengue virus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + m7G(5')pppG-RNA
m7GTP capped nucleotides 1-110. 2'-O MTase activity is seen only in glycine buffer, at pH 10, with a minimum RNA length of 20 nucleotides needed
721099
Dengue virus
S-adenosyl-L-homocysteine + m7G(5')pppGm-RNA
-
-
-
?
Other publictions for EC 2.1.1.57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
757292
Simabuco
Interactome analysis of the h ...
Homo sapiens
J. Cell. Biochem.
120
5597-5611
2019
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-
757922
Toczydlowska-Socha
Human RNA cap1 methyltransfer ...
Homo sapiens
Philos. Trans. R. Soc. Lond. B Biol. Sci.
373
20180161
2018
1
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1
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1
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758536
Quirin
The RNA capping enzyme domain ...
Flock house virus
Viruses
10
483
2018
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-
1
1
-
-
-
733878
Stewart
Structure-based identification ...
bluetongue virus
FEBS open bio
5
138-146
2015
-
-
1
1
6
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-
1
-
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4
-
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1
1
1
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1
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1
6
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1
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1
1
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720881
Szretter
2-O methylation of the viral m ...
West Nile virus
PLoS Pathog.
8
e1002698
2012
-
-
-
-
1
-
-
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-
-
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5
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-
1
1
-
-
-
721104
Ke
Short peptides derived from th ...
Severe acute respiratory syndrome-related coronavirus, Severe acute respiratory syndrome-related coronavirus WHU
Virus Res.
167
322-328
2012
1
-
1
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1
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5
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1
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720878
Decroly
Crystal structure and function ...
Severe acute respiratory syndrome-related coronavirus
PLoS Pathog.
7
e1002059
2011
1
-
-
-
31
-
-
-
-
1
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4
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1
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5
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1
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31
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1
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-
720879
Chen
Biochemical and structural ins ...
Severe acute respiratory syndrome-related coronavirus, Severe acute respiratory syndrome-related coronavirus WHU
PLoS Pathog.
7
e1002294
2011
1
-
1
1
4
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1
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6
-
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1
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4
1
4
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1
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1
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1
4
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1
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4
1
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-
704920
Selisko
Biochemical characterization o ...
Dengue virus, feline coronavirus
J. Gen. Virol.
91
112-121
2010
8
-
2
-
11
-
11
2
-
2
2
6
-
7
-
-
2
-
-
-
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6
1
10
-
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-
2
1
2
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3
8
-
2
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11
-
3
11
-
2
-
2
2
6
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2
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-
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6
1
-
-
-
-
2
1
2
-
-
-
-
-
-
-
719891
Belanger
Characterization of hMTr1, a h ...
Homo sapiens
J. Biol. Chem.
285
33037-33044
2010
-
-
1
-
1
-
2
-
1
1
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5
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1
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1
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2
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5
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1
1
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1
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1
1
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1
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1
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2
-
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1
1
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1
1
-
-
-
719892
Lugari
Molecular mapping of the RNA c ...
Severe acute respiratory syndrome-related coronavirus
J. Biol. Chem.
285
33230-33241
2010
1
1
1
-
15
-
-
-
-
-
-
-
-
5
-
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1
-
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5
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1
1
1
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15
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1
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-
-
-
-
721099
Chung
Higher catalytic efficiency of ...
Dengue virus
Virology
402
52-60
2010
-
-
1
-
-
-
2
2
-
-
-
-
-
6
-
-
1
-
-
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-
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1
-
3
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-
-
-
-
-
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2
-
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1
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2
2
-
2
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1
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-
1
-
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-
-
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-
-
-
-
721101
Zhang
Identification of aromatic ami ...
vesicular stomatitis virus
Virology
408
241-252
2010
-
-
-
-
10
-
1
-
-
-
-
-
-
1
-
-
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2
-
4
-
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-
10
-
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1
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-
2
-
-
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-
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-
-
-
671151
Mastrangelo
Preliminary characterization o ...
Meaban virus, Yokose virus
Acta Crystallogr. Sect. F
F62
768-770
2006
-
2
2
2
-
-
-
-
-
-
-
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-
8
-
-
2
-
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-
-
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4
-
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2
2
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2
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2
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672205
Li
pKa of the mRNA cap-specific 2 ...
Vaccinia virus
Biochemistry
45
907-917
2006
-
1
1
-
2
-
-
-
-
-
-
-
-
2
-
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1
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2
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1
1
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2
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1
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-
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677099
Arhin
A protein related to the vacci ...
Trypanosoma brucei
RNA
12
53-62
2006
-
2
-
-
2
-
-
-
1
-
2
-
-
9
-
-
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-
-
-
-
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5
-
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2
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2
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1
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-
675702
Li
Amino acid residues within con ...
vesicular stomatitis virus
J. Virol.
79
13373-13384
2005
-
1
1
-
7
-
-
-
-
-
-
-
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1
-
-
1
-
-
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-
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1
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1
1
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7
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1
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662252
Benarroch
A structural basis for the inh ...
Dengue virus
J. Biol. Chem.
279
35638-35643
2004
-
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1
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4
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6
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662171
Hu
Insertion of an N7-methylguani ...
Vaccinia virus
J. Biol. Chem.
278
51515-51520
2003
-
-
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1
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4
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1
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639486
Egloff
An RNA cap (nucleoside-2'-O-)- ...
Dengue virus
EMBO J.
21
2757-2768
2002
-
-
1
1
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-
-
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1
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5
-
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1
1
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3
1
1
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1
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1
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1
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1
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3
1
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639488
Latner
The vaccinia virus bifunctiona ...
Vaccinia virus
Virology
269
345-355
2000
-
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2
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4
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2
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485465
Ramadevi
Capping and methylation of mRN ...
bluetongue virus
Proc. Natl. Acad. Sci. USA
95
13537-13542
1998
-
-
1
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1
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1
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1
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1
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1
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1
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1
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1
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2
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1
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1
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639484
Lockless
Recognition of capped RNA subs ...
Vaccinia virus
Biochemistry
37
8564-8574
1998
-
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1
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5
-
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3
-
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1
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1
1
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9
-
1
1
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1
1
1
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1
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5
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3
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1
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9
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1
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1
1
1
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639487
Schnierle
Mutational analysis of a multi ...
Vaccinia virus
J. Biol. Chem.
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1994
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1
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4
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4
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1
-
-
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1
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1
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1
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4
-
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1
-
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-
1
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-
-
-
-
-
-
-
-
-
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-
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639480
Schnierle
Cap-specific mRNA (nucleoside- ...
Vaccinia virus, Vaccinia virus WR
Proc. Natl. Acad. Sci. USA
89
2897-2901
1992
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-
-
-
-
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1
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1
2
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11
-
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1
-
-
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4
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-
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-
-
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-
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1
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1
2
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1
-
-
-
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4
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-
-
-
-
-
-
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485450
Pugh
Effects of S-adenosylhomocyste ...
Vaccinia virus, Vaccinia virus WR
Biochemistry
21
1535-1541
1982
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-
-
-
-
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19
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1
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2
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11
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4
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12
-
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19
12
-
1
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2
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-
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4
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-
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639479
Langberg
Post-transcriptional modificat ...
Homo sapiens
J. Biol. Chem.
256
10054-10060
1981
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-
-
-
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1
3
2
1
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-
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1
-
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1
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-
1
1
-
10
-
-
-
-
2
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
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2
-
3
2
1
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-
-
-
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1
-
1
1
-
10
-
-
-
2
-
-
1
-
-
-
-
-
-
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639476
Barbosa
mRNA(nucleoside-2-)-methyltran ...
Vaccinia virus, Vaccinia virus WR
J. Biol. Chem.
253
7692-7697
1978
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-
-
-
-
-
-
-
1
-
3
-
-
11
-
-
1
-
-
-
1
2
4
1
-
1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
1
-
-
1
2
4
1
1
-
-
-
2
-
-
-
-
-
-
-
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639477
Barbosa
mRNA(nucleoside-2-)-methyltran ...
Vaccinia virus, Vaccinia virus WR
J. Biol. Chem.
253
7698-7702
1978
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-
-
-
-
2
4
2
1
1
-
1
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11
-
-
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1
-
-
-
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15
-
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1
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
2
1
1
-
1
-
-
-
-
-
-
-
-
15
-
1
-
2
-
1
1
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639478
Pugh
Sinefungin, a potent inhibitor ...
Vaccinia virus, Vaccinia virus WR
J. Biol. Chem.
253
4075-4077
1978
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-
-
-
-
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3
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1
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-
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11
-
-
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-
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-
-
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-
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-
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3
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-
-
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3
3
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1
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-
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-
-
-
-
-
-
-
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-
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639483
Boone
Synthesis of mRNA guanylyltran ...
Homo sapiens
J. Virol.
21
475-483
1977
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-
-
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-
-
-
-
-
-
-
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1
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1
-
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1
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3
-
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-
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-
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-
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1
-
1
-
-
3
-
-
-
-
-
-
-
-
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-
-
-
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485454
Ensinger
Modification of the 5-terminus ...
Vaccinia virus, Vaccinia virus WR
Proc. Natl. Acad. Sci. USA
72
2525-2529
1975
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-
-
-
-
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1
-
1
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-
-
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11
-
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1
-
-
-
1
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6
-
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1
-
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
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-
-
-
-
1
-
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1
-
6
-
1
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-
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1
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-