BRENDA - Enzyme Database
show all sequences of 2.1.1.57

Biochemical characterization of the (nucleoside-2'O)-methyltransferase activity of dengue virus protein NS5 using purified capped RNA oligonucleotides (7Me)GpppAC(n) and GpppAC(n)

Selisko, B.; Peyrane, F.F.; Canard, B.; Alvarez, K.; Decroly, E.; J. Gen. Virol. 91, 112-121 (2010)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2',3',5'-tri-O-acetyladenosine
about 20% increased activity at 0.1 mM
Dengue virus
2',3',5'-tri-O-acetyladenosine
about 50% increased activity at 0.1 mM
feline coronavirus
3-deazaadenosine
about 10% increased activity at 0.1 mM
feline coronavirus
5'-deoxy-5'-(methylthio)adenosine
about 75% increased activity at 0.1 mM
feline coronavirus
5'-deoxy-5'-methylthioadenosine
about 10% increased activity at 0.1 mM
Dengue virus
5'-S-isobutylthio-5'-deoxyadenosine
about 60% increased activity at 0.1 mM
feline coronavirus
ribavirin
about 10% increased activity at 0.1 mM
feline coronavirus
ribavirin triphosphate
about 20% increased activity at 0.1 mM
feline coronavirus
Cloned(Commentary)
Cloned (Commentary)
Organism
-
Dengue virus
expressed in Escherichia coli C41(DE3) cells
feline coronavirus
Engineering
Protein Variants
Commentary
Organism
D113A
about 110% substrate binding and activity compared to the wild type enzyme
feline coronavirus
D129A
about 40% substrate binding and activity compared to the wild type enzyme
feline coronavirus
D221A
about 115% substrate binding and activity compared to the wild type enzyme
feline coronavirus
D247A
about 75% substrate binding and activity compared to the wild type enzyme
feline coronavirus
E202A
about 30% substrate binding and activity compared to the wild type enzyme
feline coronavirus
F173A
about 20% substrate binding and activity compared to the wild type enzyme
feline coronavirus
K169A
about 20% substrate binding and activity compared to the wild type enzyme
feline coronavirus
K45A
about 1% substrate binding and activity compared to the wild type enzyme
feline coronavirus
W4A
about 90% substrate binding and activity compared to the wild type enzyme
feline coronavirus
Y14A
about 10% substrate binding and activity compared to the wild type enzyme
feline coronavirus
Y29A
about 15% substrate binding and activity compared to the wild type enzyme
feline coronavirus
Inhibitors
Inhibitors
Commentary
Organism
Structure
5'-S-isobutylthio-5'-deoxyadenosine
about 25% inhibition at 0.1 mM
Dengue virus
5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide triphosphate
about 40% inhibition at 0.1 mM
Dengue virus
deazaadenosine
about 5% inhibition at 0.1 mM
Dengue virus
ribavirin
about 10% inhibition at 0.1 mM
Dengue virus
ribavirin triphosphate
about 50% inhibition at 0.1 mM
Dengue virus
S-(5'-adenosyl)-L-cysteine
about 25% inhibition at 0.1 mM
feline coronavirus
S-5'-adenosyl-L-cysteine
about 5% inhibition at 0.1 mM
Dengue virus
S-adenosyl-L-homocysteine
complete inhibition at 0.1 mM
Dengue virus
S-adenosyl-L-homocysteine
about 25% inhibition at 0.1 mM
feline coronavirus
sinefungin
complete inhibition at 0.1 mM
Dengue virus
sinefungin
about 5% inhibition at 0.1 mM
feline coronavirus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000394
-
7Me-GpppA-C5
in 40 mM Tris-HCl, pH 7.5, at 30°C
Dengue virus
0.00326
-
S-adenosyl-L-methionine
in 40 mM Tris-HCl, pH 7.5, at 30°C
Dengue virus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
110% activity in the presence of 10 mM KCl, in Tris-HCl buffer (pH 7.5)
Dengue virus
Mg2+
maximum activity in the presence of 1 mM
feline coronavirus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
feline coronavirus
65000
-
gel filtration
feline coronavirus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Dengue virus
maximum 2'O-MTase activity requires the cap and at least 6 nucleotides
?
-
-
?
additional information
feline coronavirus
the enzyme shows highest activity towards 7Me-GpppA-Cn where n equals at least four, and does not methylate GpppAC5 or pppAC5
?
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-C5
feline coronavirus
-
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-C5
Dengue virus
exclusive methylation at the 2'O position
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-Cn
Dengue virus
exclusive methylation at the 2'O position
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-Cn
-
-
?
S-adenosyl-L-methionine + GpppA-Cn
Dengue virus
exclusive methylation at the 2'O position
S-adenosyl-L-homocysteine + GpppA-2'OMe-Cn
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Dengue virus
-
-
-
feline coronavirus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Dengue virus
Sepharose resin column chromatography and Superdex 200 gel filtration
feline coronavirus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
maximum 2'O-MTase activity requires the cap and at least 6 nucleotides
704920
Dengue virus
?
-
-
-
?
additional information
the enzyme shows highest activity towards 7Me-GpppA-Cn where n equals at least four, and does not methylate GpppAC5 or pppAC5
704920
feline coronavirus
?
-
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-C5
-
704920
feline coronavirus
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5
-
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-C5
exclusive methylation at the 2'O position
704920
Dengue virus
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5
-
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-Cn
exclusive methylation at the 2'O position
704920
Dengue virus
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-Cn
-
-
-
?
S-adenosyl-L-methionine + GpppA-Cn
exclusive methylation at the 2'O position
704920
Dengue virus
S-adenosyl-L-homocysteine + GpppA-2'OMe-Cn
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 35000, SDS-PAGE
feline coronavirus
Synonyms
Synonyms
Commentary
Organism
(nucleoside-2'O)-methyltransferase
part of the NS5 protein
Dengue virus
2'O-MTase
-
Dengue virus
2'O-MTase
-
feline coronavirus
nonstructural protein 16
-
feline coronavirus
nonstructural protein 16 methyltransferase
-
feline coronavirus
nonstructural protein 5 methyltransferase
-
Dengue virus
NS5MTase
the protein contains mRNA cap (guanine N-7) methyltransferase and (nucleoside-2'O)-methyltransferase activities
Dengue virus
nsp16
-
feline coronavirus
RNA (nucleoside-2'O)-methyltransferase
-
feline coronavirus
S-adenosyl-L-methionine-dependent mRNA cap MTase
-
feline coronavirus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
feline coronavirus
8
-
-
Dengue virus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
-
Dengue virus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
10
-
at basic pH, 2'O-MTase activity is practically non-existent and neither Mg2+ nor KCl is able to activate the methyl transfer at pH 10.0
Dengue virus
10
-
at basic pH, 2'O-MTase activity is practically non-existent and neither Mg2+ nor KCl is able to activate the methyl transfer at pH 10.0
feline coronavirus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.00034
-
in 40 mM Tris-HCl, pH 7.5, at 30°C
Dengue virus
S-adenosyl-L-homocysteine
0.00063
-
in 40 mM Tris-HCl, pH 7.5, at 30°C
Dengue virus
sinefungin
0.144
-
-
feline coronavirus
S-adenosyl-L-homocysteine
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2',3',5'-tri-O-acetyladenosine
about 20% increased activity at 0.1 mM
Dengue virus
2',3',5'-tri-O-acetyladenosine
about 50% increased activity at 0.1 mM
feline coronavirus
3-deazaadenosine
about 10% increased activity at 0.1 mM
feline coronavirus
5'-deoxy-5'-(methylthio)adenosine
about 75% increased activity at 0.1 mM
feline coronavirus
5'-deoxy-5'-methylthioadenosine
about 10% increased activity at 0.1 mM
Dengue virus
5'-S-isobutylthio-5'-deoxyadenosine
about 60% increased activity at 0.1 mM
feline coronavirus
ribavirin
about 10% increased activity at 0.1 mM
feline coronavirus
ribavirin triphosphate
about 20% increased activity at 0.1 mM
feline coronavirus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Dengue virus
expressed in Escherichia coli C41(DE3) cells
feline coronavirus
Engineering (protein specific)
Protein Variants
Commentary
Organism
D113A
about 110% substrate binding and activity compared to the wild type enzyme
feline coronavirus
D129A
about 40% substrate binding and activity compared to the wild type enzyme
feline coronavirus
D221A
about 115% substrate binding and activity compared to the wild type enzyme
feline coronavirus
D247A
about 75% substrate binding and activity compared to the wild type enzyme
feline coronavirus
E202A
about 30% substrate binding and activity compared to the wild type enzyme
feline coronavirus
F173A
about 20% substrate binding and activity compared to the wild type enzyme
feline coronavirus
K169A
about 20% substrate binding and activity compared to the wild type enzyme
feline coronavirus
K45A
about 1% substrate binding and activity compared to the wild type enzyme
feline coronavirus
W4A
about 90% substrate binding and activity compared to the wild type enzyme
feline coronavirus
Y14A
about 10% substrate binding and activity compared to the wild type enzyme
feline coronavirus
Y29A
about 15% substrate binding and activity compared to the wild type enzyme
feline coronavirus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.00034
-
in 40 mM Tris-HCl, pH 7.5, at 30°C
Dengue virus
S-adenosyl-L-homocysteine
0.00063
-
in 40 mM Tris-HCl, pH 7.5, at 30°C
Dengue virus
sinefungin
0.144
-
-
feline coronavirus
S-adenosyl-L-homocysteine
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5'-S-isobutylthio-5'-deoxyadenosine
about 25% inhibition at 0.1 mM
Dengue virus
5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide triphosphate
about 40% inhibition at 0.1 mM
Dengue virus
deazaadenosine
about 5% inhibition at 0.1 mM
Dengue virus
ribavirin
about 10% inhibition at 0.1 mM
Dengue virus
ribavirin triphosphate
about 50% inhibition at 0.1 mM
Dengue virus
S-(5'-adenosyl)-L-cysteine
about 25% inhibition at 0.1 mM
feline coronavirus
S-5'-adenosyl-L-cysteine
about 5% inhibition at 0.1 mM
Dengue virus
S-adenosyl-L-homocysteine
complete inhibition at 0.1 mM
Dengue virus
S-adenosyl-L-homocysteine
about 25% inhibition at 0.1 mM
feline coronavirus
sinefungin
complete inhibition at 0.1 mM
Dengue virus
sinefungin
about 5% inhibition at 0.1 mM
feline coronavirus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000394
-
7Me-GpppA-C5
in 40 mM Tris-HCl, pH 7.5, at 30°C
Dengue virus
0.00326
-
S-adenosyl-L-methionine
in 40 mM Tris-HCl, pH 7.5, at 30°C
Dengue virus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
110% activity in the presence of 10 mM KCl, in Tris-HCl buffer (pH 7.5)
Dengue virus
Mg2+
maximum activity in the presence of 1 mM
feline coronavirus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
feline coronavirus
65000
-
gel filtration
feline coronavirus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Dengue virus
maximum 2'O-MTase activity requires the cap and at least 6 nucleotides
?
-
-
?
additional information
feline coronavirus
the enzyme shows highest activity towards 7Me-GpppA-Cn where n equals at least four, and does not methylate GpppAC5 or pppAC5
?
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-C5
feline coronavirus
-
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-C5
Dengue virus
exclusive methylation at the 2'O position
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-Cn
Dengue virus
exclusive methylation at the 2'O position
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-Cn
-
-
?
S-adenosyl-L-methionine + GpppA-Cn
Dengue virus
exclusive methylation at the 2'O position
S-adenosyl-L-homocysteine + GpppA-2'OMe-Cn
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Dengue virus
Sepharose resin column chromatography and Superdex 200 gel filtration
feline coronavirus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
maximum 2'O-MTase activity requires the cap and at least 6 nucleotides
704920
Dengue virus
?
-
-
-
?
additional information
the enzyme shows highest activity towards 7Me-GpppA-Cn where n equals at least four, and does not methylate GpppAC5 or pppAC5
704920
feline coronavirus
?
-
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-C5
-
704920
feline coronavirus
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5
-
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-C5
exclusive methylation at the 2'O position
704920
Dengue virus
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5
-
-
-
?
S-adenosyl-L-methionine + 7Me-GpppA-Cn
exclusive methylation at the 2'O position
704920
Dengue virus
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-Cn
-
-
-
?
S-adenosyl-L-methionine + GpppA-Cn
exclusive methylation at the 2'O position
704920
Dengue virus
S-adenosyl-L-homocysteine + GpppA-2'OMe-Cn
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 35000, SDS-PAGE
feline coronavirus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
feline coronavirus
8
-
-
Dengue virus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
-
Dengue virus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
10
-
at basic pH, 2'O-MTase activity is practically non-existent and neither Mg2+ nor KCl is able to activate the methyl transfer at pH 10.0
Dengue virus
10
-
at basic pH, 2'O-MTase activity is practically non-existent and neither Mg2+ nor KCl is able to activate the methyl transfer at pH 10.0
feline coronavirus
Other publictions for EC 2.1.1.57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
757292
Simabuco
Interactome analysis of the h ...
Homo sapiens
J. Cell. Biochem.
120
5597-5611
2019
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1
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4
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1
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-
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-
757922
Toczydlowska-Socha
Human RNA cap1 methyltransfer ...
Homo sapiens
Philos. Trans. R. Soc. Lond. B Biol. Sci.
373
20180161
2018
1
-
1
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-
-
-
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1
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4
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1
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1
1
2
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1
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1
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1
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1
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1
1
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-
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-
-
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-
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-
758536
Quirin
The RNA capping enzyme domain ...
Flock house virus
Viruses
10
483
2018
-
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-
5
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-
-
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-
1
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1
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-
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-
1
1
-
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-
733878
Stewart
Structure-based identification ...
bluetongue virus
FEBS open bio
5
138-146
2015
-
-
1
1
6
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
1
1
-
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1
-
1
6
-
-
-
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1
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-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720881
Szretter
2-O methylation of the viral m ...
West Nile virus
PLoS Pathog.
8
e1002698
2012
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
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-
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2
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1
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-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721104
Ke
Short peptides derived from th ...
Severe acute respiratory syndrome-related coronavirus, Severe acute respiratory syndrome-related coronavirus WHU
Virus Res.
167
322-328
2012
1
-
1
-
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1
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5
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1
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2
-
5
-
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1
-
1
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1
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1
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2
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-
720878
Decroly
Crystal structure and function ...
Severe acute respiratory syndrome-related coronavirus
PLoS Pathog.
7
e1002059
2011
1
-
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31
-
-
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1
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4
-
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1
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5
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1
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31
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1
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