Literature summary for 2.1.1.57 extracted from

Li, C.; Gershon, P.D.
pKa of the mRNA cap-specific 2-O-methyltransferase catalytic lysine by HSQC NMR detection of a two-carbon probe (2006), Biochemistry, 45, 907-917.

Search for more literature for 2.1.1.57

Application

Application	Comment	Organism
analysis	characterization of the side chain pKa for a single lysine analogue within a 316-residue protein containing 21 lysines and 1678 carbon atoms at natural isotope abundance, achieved by the single reactive cysteine of a K175C mutant of VP39 modified to S-(beta-aminoethyl)cysteine (gamma-thialysine) using freshly prepared (13C)aziridine at room temperature	Vaccinia virus

Cloned(Commentary)

Cloned (Comment)	Organism
mutant C272S/K175C/R209K	Vaccinia virus

Protein Variants

Protein Variants	Comment	Organism
C272S/K175C/R209K	2-bromoethylamine reveals 64% target site modification in a overnight reaction at 37°C, unmodified mutant has no detectable methyltransferase activity, whereas the modified protein is active, exhibiting 20-30% of the specific catalytic rate of wild-type	Vaccinia virus
K175C	aziridine reveals 66.7% target site modification in a 4 h, room temperature reaction, equivalent modification level achieved in an overnight incubation using 2-bromoethylamine at 37°C, but protein losses due to aggregation during the modification reaction are, notably, negligible in the lower temperature/shorter duration reactions employed for aziridine	Vaccinia virus

Organism

Organism	UniProt	Comment	Textmining
Vaccinia virus	P07617	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + m7G-(5')pppGpApApA	-	Vaccinia virus	?	-	?

Synonyms

Synonyms	Comment	Organism
mRNA cap-specific 2'-O-methyltransferase	-	Vaccinia virus
VP39	-	Vaccinia virus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)