Cloned (Comment) | Organism |
---|---|
cloning of His-tagged N-terminal part of NS5 protein, termed NS5MTaseDV, amino acids 1-296, overexpression in Escherichia coli | Dengue virus |
Crystallization (Comment) | Organism |
---|---|
hanging drop method at room temperature, 0.1 M sodium citrate pH 5.8, 1.2 M lithium sulfate, 0.5 M ammonium sulfate, vapour diffusion for 1 week, structure analysis with and without bound nucleotide, model | Dengue virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
recombinant truncated N-terminal protein, gel filtration | Dengue virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus | P12823 | type 2, NS5 protein | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged N-terminal part of NS5 protein from E. coli | Dengue virus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA] | nucleotide binding analysis based on crystal structure | Dengue virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + GpppACCCCC | - |
Dengue virus | S-adenosyl-L-homocysteine + GpppAmCCCCC | - |
? | |
S-adenosyl-L-methionine + m7G(5')pppR-RNA | His-tagged N-terminal part of NS5 protein, termed NS5MTaseDV, amino acids 1-296, utilizes methylated and unmethylated capped RNA, but not uncapped RNA | Dengue virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? | |
S-adenosyl-L-methionine + m7GpppACCCCC | - |
Dengue virus | S-adenosyl-L-homocysteine + m7GpppAmCCCCC | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, recombinant truncated N-terminal protein, gel filtration, SDS-PAGE | Dengue virus |
Synonyms | Comment | Organism |
---|---|---|
mRNA cap (nucleoside-2'-O)-methyltransferase | i.e. NS5 | Dengue virus |