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Literature summary for 2.1.1.57 extracted from
- mRNA(nucleoside-2-)-methyltransferase from vaccinia virus. Purification and physical properties (1978), J. Biol. Chem., 253, 7692-7697.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| viral nucleocapsid | - |
Vaccinia virus | 19013 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
gel filtration | Vaccinia virus |
| 36000 | - |
sucrose density gradient centrifugation | Vaccinia virus |
| 38000 | - |
gel filtration | Vaccinia virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | - |
- |
- |
| Vaccinia virus WR | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on ADP-Sepharose | Vaccinia virus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00000233 | - |
purified enzyme | Vaccinia virus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months | Vaccinia virus |
| 4°C, several months | Vaccinia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + m7G(5')pppR-RNA | specific, in vitro methyl group acceptors are polyribonucleosides containing 7-methylguanine(5')pppG-terminals | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? |  |
| S-adenosyl-L-methionine + m7G(5')pppR-RNA | brome mosaic virus RNA ending in m7G(5')pppG as substrate | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? | |
| S-adenosyl-L-methionine + m7G(5')pppR-RNA | specific, in vitro methyl group acceptors are polyribonucleosides containing 7-methylguanine(5')pppG-terminals | Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? | |
| S-adenosyl-L-methionine + m7G(5')pppR-RNA | brome mosaic virus RNA ending in m7G(5')pppG as substrate | Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000, SDS-PAGE | Vaccinia virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vaccinia virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 8.4 | Vaccinia virus |
| 7.6 | - |
assay at | Vaccinia virus |
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