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Literature summary for 2.1.1.56 extracted from
- Development of an ELISA assay for screening inhibitors against divalent metal ion dependent alphavirus capping enzyme (2018), Virus Res., 256, 209-218 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells | Chikungunya virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D63A | inactive | Chikungunya virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aurintricarboxylic acid | - |
Chikungunya virus |  |
| Ca2+ | about 10 % residual activity at 5 mM | Chikungunya virus | |
| Cd2+ | about 17 % residual activity at 5 mM | Chikungunya virus | |
| Co2+ | about 20 % residual activity at 5 mM | Chikungunya virus | |
| Cu2+ | about 18 % residual activity at 5 mM | Chikungunya virus | |
| EDTA | 12% residual activity at 5 mM | Chikungunya virus | |
| Ni2+ | about 25 % residual activity at 5 mM | Chikungunya virus | |
| ribavirin | - |
Chikungunya virus | |
| sinefungin | - |
Chikungunya virus | |
| Zn2+ | about 30 % residual activity at 5 mM | Chikungunya virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00745 | - |
GTP | at pH 7.3 and 25°C | Chikungunya virus | |
| 0.07221 | - |
S-adenosyl-L-methionine | at pH 7.3 and 25°C | Chikungunya virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | contains Mg2+, maximum activity in the presence of Mg2+ | Chikungunya virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chikungunya virus | A0A1I9WLN6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Chikungunya virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + GTP | - |
Chikungunya virus | S-adenosyl-L-homocysteine + m7GTP | - |
? | |
| S-adenosyl-L-methionine + guanylylimidodiphosphate | - |
Chikungunya virus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Chikungunya virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alphavirus capping enzyme | - |
Chikungunya virus |
| guanosine-7-methyltransferase | - |
Chikungunya virus |
| non-structural protein 1 | - |
Chikungunya virus |
| nsP1 methyltransferase | - |
Chikungunya virus |
| nsP1 MTase | - |
Chikungunya virus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000452 | - |
GTP | at pH 7.3 and 25°C | Chikungunya virus | |
| 0.0000705 | - |
S-adenosyl-L-methionine | at pH 7.3 and 25°C | Chikungunya virus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00269 | - |
at pH 7.3 and 25°C | Chikungunya virus | sinefungin | |
| 0.00572 | - |
at pH 7.3 and 25°C | Chikungunya virus | aurintricarboxylic acid | |
| 1.18 | - |
at pH 7.3 and 25°C | Chikungunya virus | ribavirin | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 980 | - |
S-adenosyl-L-methionine | at pH 7.3 and 25°C | Chikungunya virus | |
| 6070 | - |
GTP | at pH 7.3 and 25°C | Chikungunya virus | |
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