Cloned (Comment) | Organism |
---|---|
the MTase domain, representing the N-terminal 272 amino acids of DENV-4 NS5 is cloned and purified | Dengue virus |
Protein Variants | Comment | Organism |
---|---|---|
D146A | mutant completely loses the N-7 activity (methylation of N-7 position of guanine) and 2'-O methylation activity. Mutation defective in N-7 methylation is lethal for DNV replication | Dengue virus |
E217A | mutants retains 59% of wild-type N-7 (methylation of N-7 position of guanine) activity | Dengue virus |
K181A | mutants retains 16% of wild-type N-7 (methylation of N-7 position of guanine) activity | Dengue virus |
K61A | mutants retains 34% of wild-type N-7 (methylation of N-7 position of guanine) activity | Dengue virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Dengue virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme sequentially methylates the guanine N-7 and ribose 2'-O positions of viral RNA cap. The order of two methylations is determined by the preference of 2'-O methylation for substrate m7GpppA-RNA to GpppA-RNA, and the 2'-O methylation is not absolutely dependent on the prior N-7 methylation | Dengue virus | ? | - |
? | |
S-adenosyl-L-methionine + GpppA-RNA | - |
Dengue virus | S-adenosyl-L-homocysteine + m7GpppA-RNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DENV methyltransferase | - |
Dengue virus |
DENV-4 NS5 | - |
Dengue virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Dengue virus |
General Information | Comment | Organism |
---|---|---|
malfunction | mutation defective in N-7 methylation but not 2-O'-methylation is lethal for DNV replication | Dengue virus |