Literature summary for 2.1.1.56 extracted from

Dong, H.; Ren, S.; Zhang, B.; Zhou, Y.; Puig-Basagoiti, F.; Li, H.; Shi, P.Y.
West Nile virus methyltransferase catalyzes two methylations of the viral RNA cap through a substrate-repositioning mechanism (2008), J. Virol., 82, 4295-4307.

Protein Variants

Protein Variants	Comment	Organism
E149A	the mutant shows about 1% N7 MTase activity as compared to the wild type protein	West Nile virus
E34A	the mutant shows about 70% N7 MTase activity as compared to the wild type protein	West Nile virus
F133A	the mutant shows about 99% N7 MTase activity as compared to the wild type protein	West Nile virus
H42A	the mutant shows wild type N7 MTase activity	West Nile virus
K21A	the mutant shows about 95% N7 MTase activity as compared to the wild type protein	West Nile virus
K41A	the mutant shows about 90% N7 MTase activity as compared to the wild type protein	West Nile virus
K45A	the mutant shows about 105% N7 MTase activity as compared to the wild type protein	West Nile virus
L16A	the mutant shows about 110% N7 MTase activity as compared to the wild type protein	West Nile virus
L184A	the mutant shows about 85% N7 MTase activity as compared to the wild type protein	West Nile virus
Q114A	the mutant shows about 97% N7 MTase activity as compared to the wild type protein	West Nile virus
R213A	the mutant shows about 30% N7 MTase activity as compared to the wild type protein	West Nile virus
R37A	the mutant shows about 5% N7 MTase activity as compared to the wild type protein	West Nile virus
R44A	the mutant shows about 65% N7 MTase activity as compared to the wild type protein	West Nile virus
R57A	the mutant shows about 10% N7 MTase activity as compared to the wild type protein	West Nile virus
R84A	the mutant shows about 3% N7 MTase activity as compared to the wild type protein	West Nile virus
T216A	the mutant shows wild type N7 MTase activity	West Nile virus
V55A	the mutant shows about 50% N7 MTase activity as compared to the wild type protein	West Nile virus
W87A	the mutant shows about 10% N7 MTase activity as compared to the wild type protein	West Nile virus
Y220A	the mutant shows about 30% N7 MTase activity as compared to the wild type protein	West Nile virus
Y254A	the mutant shows about 110% N7 MTase activity as compared to the wild type protein	West Nile virus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	GTP and GpppA do not inhibit N7 MTase activity	West Nile virus

Organism

Organism	UniProt	Comment	Textmining
West Nile virus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA	-	West Nile virus	S-adenosyl-L-homocysteine + m7G(5')pppR-RNA	-	?

Synonyms

Synonyms	Comment	Organism
N7 MTase	-	West Nile virus
NS5	the nonstrucutural protein NS5 has dual functions as both an N7 methyltransferase and a nucleoside 2'-O methyltransferase	West Nile virus
RNA guanine-methyltransferase	-	West Nile virus

General Information

General Information	Comment	Organism
physiological function	N7 cap methylation is essential for viral replication	West Nile virus