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Literature summary for 2.1.1.56 extracted from

  • Mao, X.; Shuman, S.
    Vaccinia virus mRNA (guanine-7-)methyltransferase: mutational effects on cap methylation and AdoHcy-dependent photo-crosslinking of the cap to the methyl acceptor site (1996), Biochemistry, 35, 6900-6910.
    View publication on PubMed
Search for more literature for 2.1.1.56

Cloned(Commentary)

Cloned (Comment) Organism
D1 fragment, amino acids 498 to 844 Vaccinia virus
expression of D1 fragment mutants in Escherichia coli Vaccinia virus
individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli Vaccinia virus

Protein Variants

Protein Variants Comment Organism
G600A in D1 fragment, amino acids 498-844, 4% activity remaining Vaccinia virus
H682A in D1 fragment, amino acids 498-844, 4% activity remaining, unable to crosslink the cap in presence of S-adenosyl-L-homocysteine Vaccinia virus
additional information exchange of 8 residues for alanine in the D1 fragment, amino acids 498-844 Vaccinia virus
Y683A in D1 fragment, amino acids 498-844, 0.05% activity remaining, unable to crosslink the cap in presence of S-adenosyl-L-homocysteine Vaccinia virus
Y683F in D1 fragment, amino acids 498-844, 4% activity remaining Vaccinia virus
Y683S in D1 fragment, amino acids 498-844, catalytically defective, unable to crosslink the cap in presence of S-adenosyl-L-homocysteine Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit Vaccinia virus
a
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] substrate binding sites Vaccinia virus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA
-
 Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Vaccinia virus