Cloned (Comment) | Organism |
---|---|
expression of VP4 protein in Spodoptera frugiperda cells | bluetongue virus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mapping of lipid-binding site, membrane binding activity of wild-type and diverse constructed mutants | bluetongue virus |
General Stability | Organism |
---|---|
cardiolipin, phosphatidylglycerol, and phosphatidylserine | bluetongue virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate | i.e. CHAPS, strong inhibition | bluetongue virus | |
deoxycholate | inactivation | bluetongue virus | |
Digitonin | strong inhibition | bluetongue virus | |
additional information | detergents prevent the association of S-adenosyl-L-methionine with the enzyme | bluetongue virus | |
N,N-bis-(3-D-gluconamidopropyl)-deoxycholamide | strong inhibition | bluetongue virus | |
n-octyl-beta-D-gluconpyranoside | i.e. octylglucoside, strong inhibition | bluetongue virus | |
N-tetradecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate | i.e. zwittergent 3-14, strong inhibition | bluetongue virus | |
Thesit | strong inhibition | bluetongue virus | |
Triton X-100 | inactivation, reversible by addition of lipids: cardiolipin, phosphatidylglycerol, and especially phosphatidylserine | bluetongue virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | lipid-binding site, membrane binding activity of wild-type and diverse mutants | bluetongue virus | 16020 | - |
viral nucleocapsid | - |
bluetongue virus | 19013 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
bluetongue virus | - |
enzyme activity belongs to VP4 protein | - |
Purification (Comment) | Organism |
---|---|
recombinant from Spodoptera frugiperda cells | bluetongue virus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] | RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit | bluetongue virus | |
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] | lipid-binding site, membrane binding activity of wild-type and diverse mutants | bluetongue virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + G(5')pppN | N: G | bluetongue virus | S-adenosyl-L-homocysteine + m7G(5')pppG | - |
? | |
S-adenosyl-L-methionine + G(5')pppN | unmethylated cap analogue, serves as in vitro substrate | bluetongue virus | S-adenosyl-L-homocysteine + m7G(5')pppG | - |
? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | - |
bluetongue virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | bluetongue virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | bluetongue virus |