Cloned (Comment) | Organism |
---|---|
- |
Datura stramonium |
Crystallization (Comment) | Organism |
---|---|
homology-based modeling of Datura stramonium spermidine synthase SPDS1 and putrescine N-methyltransferase PMT | Datura stramonium |
Protein Variants | Comment | Organism |
---|---|---|
H108L | 40% maximal turn over velocity of wild-type, Km for S-adenosyl-L-methionine is slightly elevated, but putrescine saturation appears only at very high concentrations | Datura stramonium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
S-adenosyl-L-methionine | wild-type, pH 8.0, 37°C | Datura stramonium | |
0.099 | - |
S-adenosyl-L-methionine | mutant H108L, pH 8.0, 37°C | Datura stramonium | |
0.19 | - |
putrescine | wild-type, pH 8.0, 37°C | Datura stramonium | |
3.13 | - |
putrescine | mutant H108L, pH 8.0, 37°C | Datura stramonium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Datura stramonium | Q70EW6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + putrescine | - |
Datura stramonium | S-adenosylhomocysteine + N-methylputrescine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PMT | - |
Datura stramonium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
putrescine | mutant H108L, pH 8.0, 37°C | Datura stramonium | |
1.98 | - |
putrescine | wild-type, pH 8.0, 37°C | Datura stramonium |