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Literature summary for 2.1.1.53 extracted from

  • Junker, A.; Fischer, J.; Sichhart, Y.; Brandt, W.; Draeger, B.
    Evolution of the key alkaloid enzyme putrescine N-methyltransferase from spermidine synthase (2013), Front. Plant Sci., 4, 260.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.53

Cloned(Commentary)

Cloned (Comment) Organism
-
 Datura stramonium

Crystallization (Commentary)

Crystallization (Comment) Organism
homology-based modeling of Datura stramonium spermidine synthase SPDS1 and putrescine N-methyltransferase PMT Datura stramonium

Protein Variants

Protein Variants Comment Organism
H108L 40% maximal turn over velocity of wild-type, Km for S-adenosyl-L-methionine is slightly elevated, but putrescine saturation appears only at very high concentrations Datura stramonium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 S-adenosyl-L-methionine wild-type, pH 8.0, 37°C Datura stramonium
0.099
-
 S-adenosyl-L-methionine mutant H108L, pH 8.0, 37°C Datura stramonium
0.19
-
 putrescine wild-type, pH 8.0, 37°C Datura stramonium
3.13
-
 putrescine mutant H108L, pH 8.0, 37°C Datura stramonium

Organism

Organism UniProt Comment Textmining
Datura stramonium Q70EW6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + putrescine
-
 Datura stramonium S-adenosylhomocysteine + N-methylputrescine
-
 ?

Synonyms

Synonyms Comment Organism
PMT
-
 Datura stramonium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.78
-
 putrescine mutant H108L, pH 8.0, 37°C Datura stramonium
1.98
-
 putrescine wild-type, pH 8.0, 37°C Datura stramonium