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Literature summary for 2.1.1.53 extracted from
- Molecular cloning and expression of putrescine N-methyltransferase from the hairy roots of Anisodus tanguticus (2005), Planta Med., 71, 987-989.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the deduced amino acid sequence of Anisodus tanguticus shows 92% identity with both Hyoscyamus niger and Atropa belladonna PMT | Anisodus tanguticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pETat-32a(+) vector and expressed in Escherichia coli BL21trxB(DE3) | Anisodus tanguticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 51% inhibition by 5 mM | Anisodus tanguticus |  |
| Ca2+ | 28% inhibition by 5 mM | Anisodus tanguticus | |
| Cr2+ | 31% inhibition by 5 mM | Anisodus tanguticus | |
| Cu2+ | 73% inhibition by 5 mM | Anisodus tanguticus | |
| EDTA | 23% inhibition by 5 mM | Anisodus tanguticus | |
| Fe2+ | 33% inhibition by 5 mM | Anisodus tanguticus | |
| Fe3+ | 14% inhibition by 5 mM | Anisodus tanguticus | |
| Mg2+ | 28% inhibition by 5 mM | Anisodus tanguticus | |
| Mn2+ | 26% inhibition by 5 mM | Anisodus tanguticus | |
| additional information | no enzyme activity without the addition of S-adenosyl-L-methionine | Anisodus tanguticus | |
| Ni2+ | 71% inhibition by 5 mM | Anisodus tanguticus | |
| Zn2+ | 27% inhibition by 5 mM | Anisodus tanguticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Anisodus tanguticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anisodus tanguticus | Q68HB1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by HiTrap affinity chromatography and gel filtration | Anisodus tanguticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Anisodus tanguticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + putrescine | - |
Anisodus tanguticus | S-adenosyl-L-homocysteine + N-methylputrescine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Anisodus tanguticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMT | - |
Anisodus tanguticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Anisodus tanguticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Anisodus tanguticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | - |
Anisodus tanguticus |
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Release 2023.1 (January 2023)
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