Literature summary for 2.1.1.5 extracted from

Mladkova, J.; Vanek, V.; Budesinsky, M.; Elbert, T.; Demianova, Z.; Garrow, T.A.; Jiracek, J.
Double-headed sulfur-linked amino acids as first inhibitors for betaine-homocysteine S-methyltransferase 2 (2012), J. Med. Chem., 55, 6822-6831.

Search for more literature for 2.1.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) (coexpression of human recombinant betaine-homocysteine S-methyltransferase)	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
(2S,11RS)-5-thia-2,11-diamino-8,8-dimethyldodecanedioic acid	more than 90% inhibition at 0.02 mM	Homo sapiens
(2S,11RS)-5-thia-2,11-diaminododecanedioic acid	about 60% inhibition at 0.02 mM	Homo sapiens
(2S,11S)-5,8-dithia-2,11-diaminododecanedioic acid	about 60% inhibition at 0.02 mM	Homo sapiens
(2S,5RS,8RS,11S)-5,8-dithia-2,11-diaminododecanedioic acid 5,8-dioxide	about 20% inhibition at 0.02 mM	Homo sapiens
(2S,8RS,11RS)-5-thia-2,11-diamino-8-methyldodecanedioic acid	competitive, more than 90% inhibition at 0.02 mM	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40300	-	x * 40300, 363 amino acid residues	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
stabilized by copurification with human recombinant betaine-homocysteine S-methyltransferase, chitin affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Homo sapiens	-
liver	-	Homo sapiens	-

Storage Stability

Storage Stability	Organism
stable for a minimum of one month at 4°C in a mixture with human recombinant betaine-homocysteine S-methyltransferase (copurified with human recombinant betaine-homocysteine S-methyltransferase)	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-homocysteine + S-methylmethionine	-	Homo sapiens	2 L-methionine	-	?

Subunits

Subunits	Comment	Organism
?	x * 40300, 363 amino acid residues	Homo sapiens

Synonyms

Synonyms	Comment	Organism
betaine-homocysteine S-methyltransferase 2	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000077	-	(2S,8RS,11RS)-5-thia-2,11-diamino-8-methyldodecanedioic acid	IC50 for betaine-homocysteine S-methyltransferase about 0.077 mM, pH 7.5, 37°C	Homo sapiens

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Release 2023.1 (January 2023)