Literature summary for 2.1.1.5 extracted from

Gonzalez, B.; Campillo, N.; Garrido, F.; Gasset, M.; Sanz-Aparicio, J.; Pajares, M.A.
Active-site-mutagenesis study of rat liver betaine-homocysteine S-methyltransferase (2003), Biochem. J., 370, 945-952.

Protein Variants

Protein Variants	Comment	Organism
A119G	1.9fold reduced turnover-number for betaine and L-homocysteine, 1.6fold reduced KM-value for L-homocysteine, 1.1fold decrease in Km-value for betaine	Rattus norvegicus
C186A	1.8fold reduced turnover-number for betaine and L-homocysteine, 4.3fold reduced KM-value for L-homocysteine, 1.8fold increase in Km-value for betaine	Rattus norvegicus
C186S	2.3fold reduced turnover-number for betaine and L-homocysteine, 1.4fold reduced KM-value for L-homocysteine, 111.6fold decrease in Km-value for betaine	Rattus norvegicus
D26A	2.7fold reduced turnover-number for betaine and L-homocysteine, 1.7fold reduced KM-value for L-homocysteine, 3.5fold increase in Km-value for betaine	Rattus norvegicus
D26I	67fold reduced turnover-number for betaine and L-homocysteine, maximal velocity is 6.5fold lower than the wild-type value	Rattus norvegicus
E159G	60fold reduced turnover-number for betaine and L-homocysteine, maximal velocity is 61.2fold lower than the wild-type value	Rattus norvegicus
E159K	170fold reduced turnover-number for betaine and L-homocysteine, maximal velocity is 169.7fold lower than the wild-type value	Rattus norvegicus
E21A	2.3fold reduced turnover-number for betaine and L-homocysteine, 6fold reduced KM-value for L-homocysteine, 2.4fold reduced Km-value for betaine	Rattus norvegicus
E21K	1.6fold reduced turnover-number for betaine and L-homocysteine, 1.4fold reduced KM-value for L-homocysteine, 1.7fold reduced Km-value for betaine	Rattus norvegicus
F74A	1.9fold reduced turnover-number for betaine and L-homocysteine, 1.9fold reduced KM-value for L-homocysteine, 2.9fold increase in Km-value for betaine	Rattus norvegicus
T184G	2.4fold reduced turnover-number for betaine and L-homocysteine, 3.19fold reduced KM-value for L-homocysteine, 1.6fold increase in Km-value for betaine	Rattus norvegicus
T73G	3.1fold reduced turnover-number for betaine and L-homocysteine, 2.2fold reduced KM-value for L-homocysteine, 1.3fold reduced Km-value for betaine	Rattus norvegicus
Y77A	14fold reduced turnover-number for betaine and L-homocysteine, maximal velocity is 13.7fold lower than the wild-type value	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.017	-	L-homocysteine	37°C, mutant enzyme E21A	Rattus norvegicus
0.025	-	L-homocysteine	37°C, mutant enzyme C186A	Rattus norvegicus
0.034	-	L-homocysteine	37°C, mutant enzyme T184G	Rattus norvegicus
0.049	-	L-homocysteine	37°C, mutant enzyme T73G	Rattus norvegicus
0.057	-	L-homocysteine	37°C, mutant enzyme F74A	Rattus norvegicus
0.063	-	L-homocysteine	37°C, mutant enzyme D26A	Rattus norvegicus
0.068	-	L-homocysteine	37°C, mutant enzyme A119G	Rattus norvegicus
0.073	-	L-homocysteine	37°C, mutant enzyme E21K	Rattus norvegicus
0.074	-	L-homocysteine	37°C, mutant enzyme C186S	Rattus norvegicus
0.106	-	L-homocysteine	37°C, wild-type enzyme	Rattus norvegicus
0.139	-	betaine	37°C, mutant enzyme E21A	Rattus norvegicus
0.185	-	betaine	37°C, mutant enzyme C186A	Rattus norvegicus
0.195	-	betaine	37°C, mutant enzyme E21K	Rattus norvegicus
0.206	-	betaine	37°C, mutant enzyme T186S	Rattus norvegicus
0.258	-	betaine	37°C, mutant enzym T73G	Rattus norvegicus
0.305	-	betaine	37°C, mutant enzyme A119G	Rattus norvegicus
0.333	-	betaine	37°C, wild-type enzyme	Rattus norvegicus
0.548	-	betaine	37°C, mutant enzyme T184G	Rattus norvegicus
0.969	-	betaine	37°C, mutant enzyme F74A	Rattus norvegicus
1.17	-	betaine	37°C, mutant enzyme D26A	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	O09171	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-homocysteine + betaine	-	Rattus norvegicus	L-methionine + dimethylglycine	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.000082	-	L-homocysteine	37°C, mutant enzyme E159K	Rattus norvegicus
0.000082	-	betaine	37°C, mutant enzyme E159K	Rattus norvegicus
0.00023	-	L-homocysteine	37°C, mutant enzyme E159G	Rattus norvegicus
0.00023	-	betaine	37°C, mutant enzyme E159G	Rattus norvegicus
0.001	-	L-homocysteine	37°C, mutant enzyme Y77A	Rattus norvegicus
0.001	-	betaine	37°C, mutant enzyme Y77A	Rattus norvegicus
0.0021	-	L-homocysteine	37°C, mutant enzyme D26I	Rattus norvegicus
0.0021	-	betaine	37°C, mutant enzyme D26I	Rattus norvegicus
0.0045	-	betaine	37°C, mutant enzym T73G	Rattus norvegicus
0.0045	-	L-homocysteine	37°C, mutant enzyme T73G	Rattus norvegicus
0.0051	-	L-homocysteine	37°C, mutant enzyme D26A	Rattus norvegicus
0.0051	-	betaine	37°C, mutant enzyme D26A	Rattus norvegicus
0.0058	-	L-homocysteine	37°C, mutant enzyme T184G	Rattus norvegicus
0.0058	-	betaine	37°C, mutant enzyme T184G	Rattus norvegicus
0.006	-	L-homocysteine	37°C, mutant enzyme C186S	Rattus norvegicus
0.006	-	L-homocysteine	37°C, mutant enzyme E21A	Rattus norvegicus
0.006	-	betaine	37°C, mutant enzyme E21A	Rattus norvegicus
0.006	-	betaine	37°C, mutant enzyme T186S	Rattus norvegicus
0.0072	-	L-homocysteine	37°C, mutant enzyme F74A	Rattus norvegicus
0.0072	-	betaine	37°C, mutant enzyme F74A	Rattus norvegicus
0.0075	-	L-homocysteine	37°C, mutant enzyme A119G	Rattus norvegicus
0.0075	-	betaine	37°C, mutant enzyme A119G	Rattus norvegicus
0.0076	-	L-homocysteine	37°C, mutant enzyme C186A	Rattus norvegicus
0.0076	-	betaine	37°C, mutant enzyme C186A	Rattus norvegicus
0.009	-	L-homocysteine	37°C, mutant enzyme E21K	Rattus norvegicus
0.009	-	betaine	37°C, mutant enzyme E21K	Rattus norvegicus
0.014	-	L-homocysteine	37°C, wild-type enzyme	Rattus norvegicus
0.014	-	betaine	37°C, wild-type enzyme	Rattus norvegicus