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Literature summary for 2.1.1.5 extracted from

  • Skiba, W.E.; Wells, M.S.; Mangum, J.H.; Awad, W.M.
    Betaine-homocysteine S-methyltransferase (human) (1987), Methods Enzymol., 143, 384-388.
    View publication on PubMed
Search for more literature for 2.1.1.5

General Stability

General Stability Organism
divalent cations and 2-mercaptoethanol do not stabilize during purification Homo sapiens
lyophilization inactivates Homo sapiens
substrates stabilize during purification Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,3-Dimethylbutyrate competitive to betaine Homo sapiens
Butyrate moderate, competitive to betaine Homo sapiens
Isovalerate competitive to betaine Homo sapiens
additional information no inhibition by sarcosine Homo sapiens
S-(delta-Carboxybutyl)-DL-homocysteine
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 6 * 45000, SDS-PAGE Homo sapiens
270000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Equus caballus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two forms of the enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Homo sapiens
-
kidney
-
 Equus caballus
-
liver
-
 Homo sapiens
-
liver
-
 Equus caballus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.314
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, over a year in 50% v/v glycerol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N,N-trimethylglycine + L-homocysteine
-
 Homo sapiens N,N-dimethylglycine + L-methionine
-
 ?
N,N,N-trimethylglycine + L-homocysteine
-
 Equus caballus N,N-dimethylglycine + L-methionine
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 45000, SDS-PAGE Homo sapiens