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Literature summary for 2.1.1.46 extracted from

  • Wengenmayer, H.; Ebel, J.; Grisebach, H.
    Purification and properties of a S-adenosylmethionine: isoflavone 4-O-methyltransferase from cell suspension cultures of Cicer arietinum L (1974), Eur. J. Biochem., 50, 135-143.
    View publication on PubMed
Search for more literature for 2.1.1.46

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.001 50% inhibition Cicer arietinum
Ba2+ 1 mM, 25% inhibition Cicer arietinum
Cd2+ 1 mM, complete inhibition Cicer arietinum
Cu2+ 1 mM, complete inhibition Cicer arietinum
Fe2+ 1 mM, 78% inhibition Cicer arietinum
Mn2+ 1 mM, 43% inhibition Cicer arietinum
S-adenosyl-L-homocysteine
-
 Cicer arietinum
Zn2+ 1 mM, complete inhibition Cicer arietinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 7,4'-dihydroxyisoflavone pH 8.8, 30°C Cicer arietinum
0.16
-
 S-adenosyl-L-methionine pH 8.8, 30°C Cicer arietinum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information does not require Mg2+ or other divalent cations Cicer arietinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel filtration Cicer arietinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Cicer arietinum i.e. daidzein. Methylation is the last step in the biosynthesis of 4’-methoxyisoflavones S-adenosyl-L-homocysteine + 7-hydroxy-4'-O-methylisoflavone
-
 ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone Cicer arietinum i.e. daidzein. Methylation is the last step in the biosynthesis of 4'-methoxyisoflavones S-adenosyl-L-homocysteine + 7-hydroxy-4'-O-methylisoflavone
-
 ?

Organism

Organism UniProt Comment Textmining
Cicer arietinum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cicer arietinum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Cicer arietinum
-
seedling
-
 Cicer arietinum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no methylation of: p-hydroxycinnamic acid, p-hydroxybenzoic acid, 3,4-dihydroxycinnamic acid, naringenin, (5,7,4'-trihydroxyflavanone), apigenin (5,7,4'-trihydroxyflavone), luteolin (5,7,3',4'-tetrahydroxyflavone), kaempferol (3,5,7,4'-tetrahydroxyflavone), wedelolactone (5,3',4'-trihydroxy-7-methoxycoumestone) Cicer arietinum ?
-
 ?
S-adenosyl-L-methionine + 5,7,4'trihydroxyisoflavone
-
 Cicer arietinum S-adenosyl-L-homocysteine + 5,7-dihydroxy-4'-O-methylisoflavone
-
 ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. Methylation is the last step in the biosynthesis of 4’-methoxyisoflavones Cicer arietinum S-adenosyl-L-homocysteine + 7-hydroxy-4'-O-methylisoflavone
-
 ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. Methylation is the last step in the biosynthesis of 4'-methoxyisoflavones Cicer arietinum S-adenosyl-L-homocysteine + 7-hydroxy-4'-O-methylisoflavone
-
 ?
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein. The reaction follows an ordered BiBi mechanism with S-adenosylmethionine and S-adenosylhomocysteine as leading reaction partners Cicer arietinum S-adenosyl-L-homocysteine + 7-hydroxy-4'-methoxylisoflavone
-
 ?

Synonyms

Synonyms Comment Organism
S-adenosylmethionine:isoflavone 4'-O-methyltransferase
-
 Cicer arietinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 in Tris-HCl buffer Cicer arietinum
9
-
 in glycine-NaOH buffer Cicer arietinum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 pH 7.5: about 40% of maximal activity (Tris-HCl buffer), pH 10.0: about 25% of maximal activity (glycine-NaOH buffer) Cicer arietinum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.03
-
 S-adenosyl-L-homocysteine pH 8.8, 30°C, versus S-adenosyl-L-methionine Cicer arietinum
0.3
-
 S-adenosyl-L-homocysteine pH 8.8, 30°C, versus 7,4'-dihydroxyisoflavone Cicer arietinum