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Literature summary for 2.1.1.45 extracted from

  • Senkovich, O.; Schormann, N.; Chattopadhyay, D.
    Structures of dihydrofolate reductase-thymidylate synthase of Trypanosoma cruzi in the folate-free state and in complex with two antifolate drugs, trimetrexate and methotrexate (2009), Acta Crystallogr. Sect. D, 65, 704-716.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
the enzyme in folate-free state, in complex with trimetrexate, and in complex with methotrexate is crystallized by the hanging drop vapor diffusion method, using 16-20% (v/v) 5-methylpentane-2,3-diol, 8-12% (w/v) PEG 4000, 0.1 M potassium citrate pH 6.0, or 1.5-1.7 M ammonium sulfate, 0.01 M magnesium chloride, 5% (w/v) ethylene glycol, 0.05 M Tris pH 7.5, or 17% (v/v) 5-methylpentane-2,3-diol, 10% (w/v) PEG 4000, 0.1 M potassium citrate pH 6.0 Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q27793
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
 Trypanosoma cruzi dihydrofolate + dTMP
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
DHFR-TS
-
 Trypanosoma cruzi
dihydrofolate reductase-thymidylate synthase
-
 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
N5,N10-methylenetetrahydrofolate
-
 Trypanosoma cruzi