Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | P13922 | - |
- |
Plasmodium falciparum TM4/8.2 | P13922 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Plasmodium falciparum | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Plasmodium falciparum TM4/8.2 | dihydrofolate + dTMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thymidylate synthase-dihydrofolate reductase | bifunctional enzyme | Plasmodium falciparum |
TS-DHFR | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
5,10-methylenetetrahydrofolate | D4 N-terminal tail mutant enzyme | Plasmodium falciparum | |
1.2 | - |
5,10-methylenetetrahydrofolate | wild type enzyme | Plasmodium falciparum | |
1.4 | - |
5,10-methylenetetrahydrofolate | Ala-FACE mutant enzyme with residues 284, 285, 288, 289, and 292 mutated to Ala | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
N5,N10-methylenetetrahydrofolate | - |
Plasmodium falciparum |