Application | Comment | Organism |
---|---|---|
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
Escherichia coli NM522 cells used for cloning and library construction, Escherichia coli chi2913recA lacking TS used for expression by complementation | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
2'-deoxyuridylate | mutant 362(T51S,K82Q,N171S) | Homo sapiens | |
0.001 | - |
2'-deoxyuridylate | mutant Y258F | Homo sapiens | |
0.0011 | - |
2'-deoxyuridylate | mutant 302(T53S,Y258F) | Homo sapiens | |
0.002 | - |
2'-deoxyuridylate | wild type | Homo sapiens | |
0.0026 | - |
2'-deoxyuridylate | mutant T51S | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P04818 | human | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6R)-N5,N10-methylene-5,6,7,8-tetrahydrofolate + 2'-deoxyuridylate | - |
Homo sapiens | 7,8-dihydrofolate + 5-methyl-2'-deoxyuridylate | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer | Homo sapiens |