Application | Comment | Organism |
---|---|---|
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Homo sapiens |
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
medicine | human TS mutants have potential applications in gene therapy by protecting hematopoietic progenitors from TS inhibitor toxicity, drug-resistant variants can be used to decrease the myelosuppressive side effects of TS-directed anticancer agents or to select genetically modified cells in vivo, clincal trials introduce chemoprotecting genes into hematopoietic cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
bacterial expression vector for overproduction in Escherichia coli | Homo sapiens |
Escherichia coli NM522 cells used for cloning and library construction, Escherichia coli chi2913recA lacking TS used for expression by complementation | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystallized in the I2(1)3 space group | Escherichia coli |
crystals grown by vapor diffusion method in hanging drop setup | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
2'-deoxyuridylate | wild-type | Escherichia coli | |
0.0053 | - |
2'-deoxyuridylate | mutant S167A | Escherichia coli | |
0.0081 | - |
methylenetetrahydrofolate | wild-type | Escherichia coli | |
0.0085 | - |
methylenetetrahydrofolate | wild-type | Escherichia coli | |
0.0097 | - |
5-bromo-2'-deoxyuridine | wild-type | Escherichia coli | |
0.0097 | - |
methylenetetrahydrofolate | mutant S167A | Escherichia coli | |
0.012 | - |
methylenetetrahydrofolate | mutant S167A | Escherichia coli | |
0.0339 | - |
2'-deoxyuridylate | mutant S167G | Escherichia coli | |
0.0417 | - |
2'-deoxyuridylate | mutant S167T | Escherichia coli | |
0.0426 | - |
methylenetetrahydrofolate | mutant S167G | Escherichia coli | |
0.0521 | - |
methylenetetrahydrofolate | mutant S167T | Escherichia coli | |
0.0627 | - |
methylenetetrahydrofolate | mutant S167T | Escherichia coli | |
0.0723 | - |
5-bromo-2'-deoxyuridine | mutant S167A | Escherichia coli | |
0.0981 | - |
2'-deoxyuridylate | mutant S167C | Escherichia coli | |
0.13 | - |
methylenetetrahydrofolate | mutant S167C | Escherichia coli | |
0.514 | - |
5-bromo-2'-deoxyuridine | mutant S167G | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A884 | - |
- |
Homo sapiens | - |
human colorectal tumor cell line C | - |
Lacticaseibacillus casei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP | - |
Escherichia coli | 7,8-dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + 5-bromo-2'-deoxyuridine | - |
Escherichia coli | ? | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation does not require methylenetetrahydrofolate as cosubstrate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Lacticaseibacillus casei |
dimer | - |
Escherichia coli |
dimer | 2 * 35000, SDS-PAGE | Homo sapiens |