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Literature summary for 2.1.1.45 extracted from

  • Changchien, L.M.; Garibian, A.; Frasca, V.; Lobo, A.; Maley, G.F.; Maley, F.
    High-Level expression of Escherichia coli and Bacillus subtilis thymidylate synthases (2000), Protein Expr. Purif., 19, 265-270.
    View publication on PubMed
Search for more literature for 2.1.1.45

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Bacillus subtilis
dithiothreitol
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Bacillus subtilis
-
 dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis
-
 Escherichia coli

Storage Stability

Storage Stability Organism
4°C enzyme can be maintained for at least 4 months with little or no loss in activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
 Bacillus subtilis dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP
-
 Bacillus subtilis dihydrofolate + dTMP
-
 r
5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
 fairly stable, incubation for 10 min results in loss of 50% enzyme activity Escherichia coli
50
-
 fairly stable, incubation for 10 min results in loss of 50% enzyme activity Bacillus subtilis