Application | Comment | Organism |
---|---|---|
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
2'-deoxyuridylate | wild-type | Escherichia coli | |
0.0012 | - |
2'-deoxyuridylate | mutant Y4F | Escherichia coli | |
0.0014 | - |
2'-deoxyuridylate | mutant Y4I, Y4V | Escherichia coli | |
0.003 | - |
2'-deoxyuridylate | mutant Y4H | Escherichia coli | |
0.008 | - |
methylenetetrahydrofolate | mutant Y4F | Escherichia coli | |
0.01 | - |
methylenetetrahydrofolate | wild-type | Escherichia coli | |
0.013 | - |
methylenetetrahydrofolate | mutant Y4H | Escherichia coli | |
0.015 | - |
methylenetetrahydrofolate | mutant Y4V | Escherichia coli | |
0.018 | - |
methylenetetrahydrofolate | mutant Y4I | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
human | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP | - |
Escherichia coli | 7,8-dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Homo sapiens | dihydrofolate + dTMP | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
N5,N10-methylenetetrahydrofolate | - |
Homo sapiens |