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Literature summary for 2.1.1.45 extracted from

  • Finer-Moore, J.S.; Liu, L.; Schafmeister, C.E.; Birdsall, D.L.; Mau, T.; Santi, D.V.; Stroud, R.M.
    Partitioning roles of side chains in affinity, orientation, and catalysis with structures for mutant complexes: Asparagine-229 in thymidylate synthase (1996), Biochemistry, 35, 5125-5136.
    View publication on PubMed
Search for more literature for 2.1.1.45

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 2'-deoxyuridylate wild-type Lacticaseibacillus casei
0.01
-
 5,10-methylenetetrahydrofolate wild-type Lacticaseibacillus casei
0.017
-
 2'-deoxyuridylate mutant TS N229C Lacticaseibacillus casei
0.05
-
 2'-deoxyuridylate mutant TS N229D Lacticaseibacillus casei
0.092
-
 5,10-methylenetetrahydrofolate mutant TS N229C Lacticaseibacillus casei
0.092
-
 5,10-methylenetetrahydrofolate mutant TS N229D Lacticaseibacillus casei
0.15
-
 2'-deoxycytidine 5'-monophosphate mutant TS N229D Lacticaseibacillus casei
0.17
-
 5,10-methylenetetrahydrofolate mutant TS N229D, dCMP as substrate Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei TS N229D
-
 dihydrofolate + dTMP
-
 ?

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
 and strain TS N229C
-
Lacticaseibacillus casei TS N229D
-
 and strain TS N229C
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate only mutant TS N229D Lacticaseibacillus casei dihydrofolate + cytidine phosphate
-
 ?
5,10-methylenetetrahydrofolate + 2'-deoxycytidine 5'-monophosphate only mutant TS N229D Lacticaseibacillus casei TS N229D dihydrofolate + cytidine phosphate
-
 ?
5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei TS N229D dihydrofolate + dTMP
-
 ?
5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei TS N229D dihydrofolate + dTMP
-
 r
additional information dCMP is no substrate, exception mutant TS N229D Lacticaseibacillus casei ?
-
 ?
additional information dCMP is no substrate, exception mutant TS N229D Lacticaseibacillus casei TS N229D ?
-
 ?