Application | Comment | Organism |
---|---|---|
medicine | enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs | Lacticaseibacillus casei |
Cloned (Comment) | Organism |
---|---|
Escherichia coli chi2913 transformed with pKPTSd containing the complete coding sequence for Lactobacillus casei TS | Lacticaseibacillus casei |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Lacticaseibacillus casei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridine 5'-monophosphate | - |
Lacticaseibacillus casei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000 | Lacticaseibacillus casei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Lacticaseibacillus casei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | 3 | - |
Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | 2 * 36000 | Lacticaseibacillus casei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | 35 | - |
Lacticaseibacillus casei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
(+)-methylenetetrahydrofolate | - |
Escherichia coli | |
(+)-methylenetetrahydrofolate | - |
Lacticaseibacillus casei |