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Literature summary for 2.1.1.45 extracted from
- Purification and characterization of thymidylate synthetase from rat regenerating liver (1987), Biochim. Biophys. Acta, 924, 297-302.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-deoxyuridine | - |
Rattus norvegicus |  |
| 5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Rattus norvegicus | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
| dTMP | - |
Rattus norvegicus | |
| methopterin | - |
Homo sapiens | |
| methopterin | - |
Rattus norvegicus | |
| Procion blue | - |
Rattus norvegicus | |
| UDP-glucuronate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0068 | - |
2'-deoxyuridylate | - |
Rattus norvegicus | |
| 0.065 | - |
5,10-methylenetetrahydrofolate | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Rattus norvegicus |
| 68000 | - |
gel filtration | Rattus norvegicus |
| 68000 | - |
gel filtration | Saccharomyces cerevisiae |
| 69000 | - |
Ehrlich ascites carcinoma cells | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Rattus norvegicus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Saccharomyces cerevisiae | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Rattus norvegicus Wistar | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
neoplastic leukemic CCRF-CEM cells | - |
| Lacticaseibacillus casei | - |
- |
- |
| Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus Wistar | - |
- |
- |
| Saccharomyces cerevisiae | - |
bakers' yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
- |
Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, enzyme retains activity for about 3 weeks when stored with 20 mM dithiothreitol and 0.1 mM dUMP, without dithiothreitol purified enzyme is inactivated totally in 2 days, without dUMP enzyme keeps 50% activity after 1 week | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6S)-tetrahydropteroylglutamate + 2-deoxyuridylate | - |
Rattus norvegicus | 7,8-dihydropteroylglutamate + thymidine phosphate | - |
? | |
| (6S)-tetrahydropteroylglutamate + 2-deoxyuridylate | - |
Rattus norvegicus Wistar | 7,8-dihydropteroylglutamate + thymidine phosphate | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus Wistar | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus Wistar | dihydrofolate + dTMP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Mus musculus |
| dimer | - |
Homo sapiens |
| dimer | - |
Saccharomyces cerevisiae |
| dimer | - |
Lacticaseibacillus casei |
| dimer | 2 * 35000, SDS-PAGE | Rattus norvegicus |
| monomer | 1 * 69000, Ehrlich ascites carcinoma cells | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.583 | - |
2'-deoxyuridine | - |
Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8 | half-maximal activity at pH 6.3 | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | 70% maximal activity at either pH 7.0 or 9.0, half-maximal activity at pH 6.3 | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000005 | - |
5-fluoro-2'-deoxyuridylic acid | - |
Rattus norvegicus | |
| 0.0001 | - |
Procion blue | variable substrate 2'-deoxyuridine | Rattus norvegicus | |
| 0.00022 | - |
Procion blue | variable substrate 5,10-methylenetetrahydropteroylglutamate | Rattus norvegicus | |
| 0.023 | - |
methopterin | - |
Rattus norvegicus | |
| 0.07 | - |
dTMP | variable substrate 2'-deoxyuridine | Rattus norvegicus | |
| 0.073 | - |
methopterin | variable substrate 2'-deoxyuridine | Rattus norvegicus | |
| 0.24 | - |
dTMP | variable substrate 5,10-methylenetetrahydropteroylglutamate | Rattus norvegicus | |
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