Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-bromo-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
5-fluoro-2'-deoxyuridylic acid | - |
Enterococcus faecalis | |
5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
aminopterin | - |
Saccharomyces cerevisiae | |
Cibacron blue | - |
Saccharomyces cerevisiae | |
dTMP | - |
Saccharomyces cerevisiae | |
methotrexate | - |
Homo sapiens | |
methotrexate | - |
Saccharomyces cerevisiae | |
thymidine phosphate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
2'-deoxyuridylate | - |
Saccharomyces cerevisiae | |
0.07 | - |
(-)-5,10-methylene-tetrahydropteroylglutamate | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Saccharomyces cerevisiae |
65000 | - |
gel filtration, velocity sedimentation electrophoresis | Saccharomyces cerevisiae |
68000 | - |
gel filtration | Saccharomyces cerevisiae |
69000 | - |
Ehrlich ascites carcinoma cells | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Gallus gallus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Saccharomyces cerevisiae | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Enterococcus faecalis | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Gallus gallus | - |
chicken | - |
Homo sapiens | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
Saccharomyces cerevisiae | - |
bakers' yeast | - |
Saccharomyces cerevisiae | - |
haploid strain | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blast cell | - |
Homo sapiens | - |
embryo | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Enterococcus faecalis | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Enterococcus faecalis | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mus musculus |
dimer | - |
Homo sapiens |
dimer | - |
Lacticaseibacillus casei |
dimer | 2 * 30000, SDS-PAGE | Saccharomyces cerevisiae |
monomer | 1 * 69000, Ehrlich ascites carcinoma cells | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.2 | - |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.5 | half-maximal activity at either pH 5.8 or pH 8.5 | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
Cibacron blue | - |
Saccharomyces cerevisiae | |
0.000005 | - |
5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
0.01 | - |
5-bromo-2'-deoxyuridine 5'-monophosphate | - |
Saccharomyces cerevisiae | |
0.015 | - |
aminopterin | - |
Saccharomyces cerevisiae | |
0.02 | - |
methotrexate | - |
Saccharomyces cerevisiae | |
0.02 | - |
aminopterin | - |
Saccharomyces cerevisiae | |
0.08 | - |
thymidine phosphate | - |
Saccharomyces cerevisiae | |
0.1 | - |
dTMP | - |
Saccharomyces cerevisiae | |
0.12 | - |
thymidine phosphate | - |
Saccharomyces cerevisiae |