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Literature summary for 2.1.1.45 extracted from
- TMP synthetase from Lactobacillus casei (1978), Methods Enzymol., 51, 90-97.
General Stability
| General Stability | Organism |
|---|---|
| thawing of frozen enzyme leads to irreversible precipitation of the protein and total loss of activity, no successfully lyophilization possible | Lacticaseibacillus casei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-hydroxyethyl disulfide | - |
Lacticaseibacillus casei |  |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Lacticaseibacillus casei | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
| cyanide | - |
Lacticaseibacillus casei | |
| H2O2 | - |
Lacticaseibacillus casei | |
| iodoacetamide | - |
Lacticaseibacillus casei | |
| N-ethylmaleimide | - |
Lacticaseibacillus casei | |
| p-chloromercuribenzoate | - |
Lacticaseibacillus casei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0051 | - |
2'-deoxyuridylate | in presence of 10 mM Mg2+ | Lacticaseibacillus casei | |
| 0.0068 | - |
2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
| 0.012 | - |
5,10-methylenetetrahydrofolate | in presence of 10 mM Mg2+ | Lacticaseibacillus casei | |
| 0.032 | - |
5,10-methylenetetrahydrofolate | - |
Lacticaseibacillus casei | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | in Tris-acetate buffers 10 mM enhances enzyme activity by some 30% | Lacticaseibacillus casei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Lacticaseibacillus casei |
| 70000 | - |
gel filtration | Lacticaseibacillus casei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
var. rhamnosus, ATCC 7469 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lacticaseibacillus casei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
- |
Lacticaseibacillus casei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-5°C, enzyme is stable over a period of months in presence of 10 mM 2-mercaptoethanol in capped vessels layered with argon | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + uridine 5'-phosphate | - |
Lacticaseibacillus casei | dihydrofolate + TMP | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Lacticaseibacillus casei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
UMP as substrate | Lacticaseibacillus casei |
| 6.5 | 6.8 | - |
Lacticaseibacillus casei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| (+)-methylenetetrahydrofolate | - |
Lacticaseibacillus casei | |
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Release 2023.1 (January 2023)
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