Any feedback?
Literature summary for 2.1.1.45 extracted from
- Deoxyribonucleotide biosynthesis in green algae: characterization of thymidylate synthase-dihydrofolate reductase in Scenedesmus obliquus (1987), Arch. Biochem. Biophys., 256, 244-252.
General Stability
| General Stability | Organism |
|---|---|
| -80°C, storage in 30% glycerol stabilizes enzyme | Tetradesmus obliquus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-fluoro-2'-deoxyuridylic acid | - |
Daucus carota |  |
| 5-fluoro-2'-deoxyuridylic acid | - |
Leishmania major | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Tetradesmus obliquus | |
| Arabinouridylate | - |
Tetradesmus obliquus | |
| Hydroxyurea | supresses thymidilate synthase in growing algae, but no effect on purified enzyme | Tetradesmus obliquus | |
| methotrexate | - |
Saccharomyces cerevisiae | |
| methotrexate | - |
Tetradesmus obliquus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Tetradesmus obliquus |
| 56000 | - |
2 * 56000 | Leishmania major |
| 100000 | - |
sedimentation in sucrose density gradients | Tetradesmus obliquus |
| 110000 | - |
- |
Leishmania major |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Chlamydomonas reinhardtii | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Tetradesmus obliquus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Saccharomyces cerevisiae | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Nicotiana tabacum | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Daucus carota | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Leishmania major | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Abies sp. | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Abies sp. | - |
pine | - |
| Bos taurus | - |
calf | - |
| Chlamydomonas reinhardtii | - |
- |
- |
| Daucus carota | - |
carrot, wild and culture form | - |
| Leishmania major | - |
- |
- |
| Mus musculus | - |
- |
- |
| Nicotiana tabacum | - |
tobacco | - |
| Saccharomyces cerevisiae | - |
bakers' yeast | - |
| Tetradesmus obliquus | - |
unicellular green algae | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tetradesmus obliquus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Mus musculus | - |
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0257 | - |
- |
Tetradesmus obliquus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, storage in 30% glycerol stabilizes enzyme | Tetradesmus obliquus |
| 5°C half-life in buffered solution only 2 days | Tetradesmus obliquus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Chlamydomonas reinhardtii | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Chlamydomonas reinhardtii | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Tetradesmus obliquus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Tetradesmus obliquus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Nicotiana tabacum | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Nicotiana tabacum | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Daucus carota | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Daucus carota | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Abies sp. | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Abies sp. | dihydrofolate + dTMP | - |
r | |
| additional information | bifunctional enzyme thymidilate synthase-dihydrofolate reductase, not possible to separate the 2 activities | Tetradesmus obliquus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Mus musculus |
| dimer | - |
Saccharomyces cerevisiae |
| dimer | - |
Bos taurus |
| dimer | 2 * 50000, SDS-PAGE | Tetradesmus obliquus |
| dimer | 2 * 56000 | Leishmania major |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Tetradesmus obliquus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Tetradesmus obliquus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | activity is negligible below pH 6.0 and above pH 9.0 | Tetradesmus obliquus |
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Release 2023.1 (January 2023)
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