Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.44 extracted from

  • Vit, A.; Misson, L.; Blankenfeldt, W.; Seebeck, F.
    Ergothioneine biosynthetic methyltransferase EgtD reveals the structural basis of aromatic amino acid betaine biosynthesis (2015), ChemBioChem, 16, 119-125 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of EgtD as an apoprotein (1.75 A resolution), as a binary complex with N-alpha-dimethylhistidine (1.9 A), and as a ternary complex with N-alpha-dimethylhistidine and SAH (1.5 A) Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
E285A active site mutation, change the substrate specificity and transforms the histidine-methyltransferase towards a tryptophan-methyltransferase Mycolicibacterium smegmatis
M252A/E285A active site mutation, change the substrate specificity and transforms the histidine-methyltransferase towards a tryptophan-methyltransferase Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + L-histidine Mycolicibacterium smegmatis
-
3 S-adenosyl-L-homocysteine + hercynine
-
?
3 S-adenosyl-L-methionine + L-histidine Mycolicibacterium smegmatis ATCC 700084
-
3 S-adenosyl-L-homocysteine + hercynine
-
?
S-adenosyl-L-methionine + L-histidine Mycolicibacterium smegmatis
-
S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine
-
?
S-adenosyl-L-methionine + L-histidine Mycolicibacterium smegmatis ATCC 700084
-
S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine
-
?
S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine Mycolicibacterium smegmatis
-
S-adenosyl-L-homocysteine + hercynine
-
?
S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine Mycolicibacterium smegmatis ATCC 700084
-
S-adenosyl-L-homocysteine + hercynine
-
?
S-adenosyl-L-methionine + Nalpha-methyl-L-histidine Mycolicibacterium smegmatis
-
S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine
-
?
S-adenosyl-L-methionine + Nalpha-methyl-L-histidine Mycolicibacterium smegmatis ATCC 700084
-
S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine
-
?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0R5M8
-
-
Mycolicibacterium smegmatis ATCC 700084 A0R5M8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + L-histidine
-
Mycolicibacterium smegmatis 3 S-adenosyl-L-homocysteine + hercynine
-
?
3 S-adenosyl-L-methionine + L-histidine
-
Mycolicibacterium smegmatis ATCC 700084 3 S-adenosyl-L-homocysteine + hercynine
-
?
additional information EgtD is a four- and 70-times stronger binder of Nalpha-methylhistidine and N-alpha-dimethylhistidine, respectively, than of histidine Mycolicibacterium smegmatis ?
-
-
additional information EgtD is a four- and 70-times stronger binder of Nalpha-methylhistidine and N-alpha-dimethylhistidine, respectively, than of histidine Mycolicibacterium smegmatis ATCC 700084 ?
-
-
S-adenosyl-L-methionine + DOPA poor substrate Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + L-histidine
-
Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine
-
?
S-adenosyl-L-methionine + L-histidine
-
Mycolicibacterium smegmatis ATCC 700084 S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine
-
?
S-adenosyl-L-methionine + L-phenylalanine poor substrate Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + L-tryptophan poor substrate Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + L-tyrosine poor substrate Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine
-
Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + hercynine
-
?
S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine
-
Mycolicibacterium smegmatis ATCC 700084 S-adenosyl-L-homocysteine + hercynine
-
?
S-adenosyl-L-methionine + Nalpha-methyl-L-histidine
-
Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine
-
?
S-adenosyl-L-methionine + Nalpha-methyl-L-histidine
-
Mycolicibacterium smegmatis ATCC 700084 S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine
-
?

Synonyms

Synonyms Comment Organism
EgtD
-
Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
L-histidine mutant E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
0.23
-
Nalpha-methyl-L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
0.43
-
Nalpha,Nalpha-dimethyl-L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
0.58
-
L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0011
-
L-tyrosine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
0.0019
-
L-histidine mutant M252A/E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
0.002
-
L-tryptophan wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
0.0024
-
L-phenylalanine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
0.017
-
L-tyrosine mutant M252A/E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
0.04
-
L-histidine mutant E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
0.1
-
L-phenylalanine mutant M252A/E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
0.4
-
L-tryptophan mutant E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
5.3
-
L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
5.5
-
L-tryptophan mutant M252A/E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
13
-
Nalpha-methyl-L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
17
-
Nalpha,Nalpha-dimethyl-L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis