Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Citrus depressa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Citrus depressa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
gel filtration | Citrus depressa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus depressa | A0A125T1T5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Citrus depressa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 S-adenosyl-L-methionine + quercetin | - |
Citrus depressa | 4 S-adenosyl-L-homocysteine + 3,3',5,7-tetra-O-methyl-quercetin | - |
? | |
additional information | enzyme shows methylation activity for the 3-, 5-, 6-, and 7-hydroxyl groups of flavones. The highest activity is observed for 3-hydroxyflavone (flavonol), followed by 7-hydroxyflavone with a relative activity of 15.6% of that of 3-hydroxyflavone and 5-hydroxyflavone with that of 13.5%. Very weak activity is observed for 6-hydroxyflavone and no activity for 3'- or 4'-hydroxyflavone and 7-methoxy-8-hydroxyflavone. FOMT5 does not exhibit a sequential methylation order for 3-, 5-, and 7-hydroxyl groups of quercetin. No detection of polymethylated products from naringenin, (-)-epicatechin, and equol, and cyanidin is not a substrate | Citrus depressa | ? | - |
- |
|
S-adenosyl-L-methionine + (-)-epicatechin | - |
Citrus depressa | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (2S)-naringenin | - |
Citrus depressa | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + equol | - |
Citrus depressa | S-adenosyl-L-homocysteine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE, recombinant protein including His-tag | Citrus depressa |
Synonyms | Comment | Organism |
---|---|---|
FOMT5 | - |
Citrus depressa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Citrus depressa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Citrus depressa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Citrus depressa | calculated from sequence | - |
5.8 |