Protein Variants | Comment | Organism |
---|---|---|
Y110F | no significant change in products | Chlamydomonas reinhardtii |
Y110L | remodeling the active site to alter the electronics results in delayed timing of the hydride migration from methyl attack of the DELTA24-bond, that thereby produces metabolic switching of product ratios in favor of DELTA25(27)-olefins or impairs the second C1-transfer activity | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8IJ34 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
24(28)-methylenecycloartanol + S-adenosyl-L-methionine | - |
Chlamydomonas reinhardtii | ? + S-adenosyl-L-homocysteine | ratio of DELTA25(27) to DELTA24(28)-product for wild-type is 100:0, 35% conversion | ? | |
24(28)-methylenelophenol + S-adenosyl-L-methionine | - |
Chlamydomonas reinhardtii | ? | - |
? | |
cycloartenol + S-adenosyl-L-methionine | - |
Chlamydomonas reinhardtii | 24(28)-methylenecycloartanol + S-adenosyl-L-homocysteine | ratio of DELTA25(27) to DELTA24(28)-product for wild-type is 57:43, 70% conversion | ? | |
lanosterol + S-adenosyl-L-methionine | - |
Chlamydomonas reinhardtii | 24beta-methyllanosta-8,25(27)-enol + 24(28)-methylenelanosterol + S-adenosyl-L-homocysteine | products are 55% 24beta-methyllanosta-8,25(27)-enol, 38% 24(28)-methylenelanosterol (eburicol), and 7% 24beta-ethyllanosta-8,25(27)-enol | ? | |
additional information | enzyme catalyzes a sterol methylation pathway by the algal DELTA25(27)-olefin route, where methylation proceeds by a conserved SN2 reaction and de-protonation proceeds from the pro-Z methyl group on lanosterol corresponding to C27 | Chlamydomonas reinhardtii | ? | - |
? | |
obtusifoliol + S-adenosyl-L-methionine | - |
Chlamydomonas reinhardtii | ? + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Chlamydomonas reinhardtii | fecosterol + S-adenosyl-L-homocysteine | ratio of DELTA25(27) to DELTA24(28)-product for wild-type is 28:72, 70% conversion | ? |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
24(28)-methylenecycloartanol | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.019 | - |
obtusifoliol | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.02 | - |
24(28)-methylenelophenol | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.022 | - |
Zymosterol | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.024 | - |
lanosterol | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii | |
0.025 | - |
Cycloartenol | pH not specified in the publication, temperature not specified in the publication | Chlamydomonas reinhardtii |