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Literature summary for 2.1.1.41 extracted from

  • Goto, Y.; Bogatzki, L.Y.; Bertholet, S.; Coler, R.N.; Reed, S.G.
    Protective immunization against visceral leishmaniasis using Leishmania sterol 24-C-methyltransferase formulated in adjuvant (2007), Vaccine, 25, 7450-7458.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.41

Application

Application Comment Organism
medicine the results indicate that SMT/MPL-SE can be an effective vaccine candidate for use against visceral leishmaniasis Leishmania infantum
medicine vaccine development, visceral leishmaniasis Leishmania infantum

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Leishmania infantum
pET-28a vector, expressed in Escherichia coli Rosetta, recombinant protein Leishmania infantum

Protein Variants

Protein Variants Comment Organism
additional information C57BL/6 mice immunized with a vaccine candidate consisting of 24-c-methyltransferase as an antigene formulated monophosphoryl lipid A (MPL-SE) show Ag-specific Th1 immune responses characterized by robust production of IFN-gamma upon specific Ag re-exposure in vitro. Upon challenge with Leishmania infantum, mice immunized with SMT plus MPL-SE show significant lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone Leishmania infantum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39800
-
 predicted from sequence, SDS-PAGE Leishmania infantum

Organism

Organism UniProt Comment Textmining
Leishmania infantum
-
-
-
Leishmania infantum A4IDL2 cloning of SMT protein of
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, SDS-PAGE Leishmania infantum
using Ni-NTA-chromatography Leishmania infantum

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
 Leishmania infantum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cloning of SMT gene, sequence homologies and Western Blot comparable between Leishmania donovani, Leishmania infantum and Leishmania major, serological evaluation revealed that SMT is recognized by visceral leishmaniasis (VL) patients, immunized mice shown to have lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone Leishmania infantum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme involved in biosynthesis of ergosterol, which is a target molecule of leishmanicidal and fungicidal amphotericin B, used for vaccine development Leishmania infantum ?
-
 ?

Synonyms

Synonyms Comment Organism
SMT
-
 Leishmania infantum
SMT protein
-
 Leishmania infantum
sterol 24-C-methyltransferase
-
 Leishmania infantum