Literature summary for 2.1.1.41 extracted from

Jayasimha, P.; Nes, W.D.
Photoaffinity labeling and mutational analysis of 24-C-sterol methyltransferase defines the AdoMet binding site (2008), Lipids, 43, 681-693.

Search for more literature for 2.1.1.41

Protein Variants

Protein Variants	Comment	Organism
C128L	no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.028, photolabelled: value below 1%	Saccharomyces cerevisiae
C128S	Km (mM) (substrate: AdoMet): 30, kcat (1/sec) (substrate: AdoMet): 0.005, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.008	Saccharomyces cerevisiae
D125L	no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.006, Kd (mM) (AdoMet): 0.012, photolabelled: 30%	Saccharomyces cerevisiae
D152L	no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.014, photolabelled: 35%	Saccharomyces cerevisiae
additional information	by photoaffinity labeling and mutational analysis the AdoMet binding site is defined. Results indicate that one or both of Cys128 and Pro133 are covalently bound to AdoMet	Saccharomyces cerevisiae
P133L	no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.015, Kd (mM) (AdoMet): no binding, photolabelled: value below 1%	Saccharomyces cerevisiae
Y153F	Km (mM) (substrate: AdoMet): 0.016, kcat (1/sec) (substrate: AdoMet): 0.003, Kd (mM) (zymosterol): 0.007, Kd (mM) (AdoMet): 0.013, photolabelled: 55%	Saccharomyces cerevisiae
Y153L	Km (mM) (substrate: AdoMet): 0.017, kcat (1/sec) (substrate: AdoMet): 0.01, Kd (mM) (zymosterol): 0.004, Kd (mM) (AdoMet): 0.004	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-deoxy-5'-(methylthio)adenosine	AdoMet analog, competitive inhibitor	Saccharomyces cerevisiae
S-isobutyladenosine	AdoMet analog, competitive inhibitor	Saccharomyces cerevisiae
sinefungin	AdoMet analog, competitive inhibitor	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.016	-	AdoMet	mutant Tyr153Phe	Saccharomyces cerevisiae
0.017	-	AdoMet	wild-type	Saccharomyces cerevisiae
0.017	-	AdoMet	mutant Tyr153Leu	Saccharomyces cerevisiae
0.03	-	AdoMet	mutant Cys128Ser	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + zymosterol	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + fecosterol	-	?

Synonyms

Synonyms	Comment	Organism
24-C-sterol methyltransferase	-	Saccharomyces cerevisiae
SMT	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.003	-	AdoMet	mutant Tyr153Phe	Saccharomyces cerevisiae
0.005	-	AdoMet	mutant Cys128Ser	Saccharomyces cerevisiae
0.01	-	AdoMet	wild-type	Saccharomyces cerevisiae
0.01	-	AdoMet	mutant Tyr153Leu	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
AdoMet	-	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.013	-	sinefungin	-	Saccharomyces cerevisiae
0.022	-	5'-deoxy-5'-(methylthio)adenosine	-	Saccharomyces cerevisiae
0.058	-	S-isobutyladenosine	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)