Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharomyces cerevisiae |
expressed in Escherichia coli, recombinant protein, site-directed mutagenesis | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
A193L | no activity | Saccharomyces cerevisiae |
A193L | no enzyme activity | Saccharomyces cerevisiae |
A193S | 95% relative enzyme activity | Saccharomyces cerevisiae |
A193S | relative activity: 95% | Saccharomyces cerevisiae |
A196L | 73% relative enzyme activity | Saccharomyces cerevisiae |
A196L | relative activity: 73% | Saccharomyces cerevisiae |
A200L | 4% relative enzyme activity | Saccharomyces cerevisiae |
A200L | relative activity: 4% | Saccharomyces cerevisiae |
A221L | 25% relative enzyme activity | Saccharomyces cerevisiae |
A221L | relative activity: 25% | Saccharomyces cerevisiae |
A350L | 100% relative enzyme activity | Saccharomyces cerevisiae |
A350L | relative activity: 100% | Saccharomyces cerevisiae |
C128L | no activity | Saccharomyces cerevisiae |
C128L | no enzyme activity | Saccharomyces cerevisiae |
C198L | 95% relative enzyme activity | Saccharomyces cerevisiae |
C198L | relative activity: 95% | Saccharomyces cerevisiae |
C198V | 16% relative enzyme activity | Saccharomyces cerevisiae |
C198V | relative activity: 16% | Saccharomyces cerevisiae |
D125L | no activity | Saccharomyces cerevisiae |
D152L | no activity | Saccharomyces cerevisiae |
D189L | relative activity: 91% | Saccharomyces cerevisiae |
D229L | relative activity: 100% | Saccharomyces cerevisiae |
D276L | no activity | Saccharomyces cerevisiae |
D65L | relative activity: 100% | Saccharomyces cerevisiae |
D79L | relative activity: 81% | Saccharomyces cerevisiae |
E108L | relative activity: 34% | Saccharomyces cerevisiae |
E195L | no activity | Saccharomyces cerevisiae |
E209L | relative activity: 79% | Saccharomyces cerevisiae |
E224L | relative activity: 74% | Saccharomyces cerevisiae |
E246L | relative activity: 6% | Saccharomyces cerevisiae |
E64L | relative activity: 100% | Saccharomyces cerevisiae |
E68L | no activity | Saccharomyces cerevisiae |
E82D | 55% relative enzyme activity | Saccharomyces cerevisiae |
E82D | relative activity: 55% | Saccharomyces cerevisiae |
E82L | relative activity: 99% | Saccharomyces cerevisiae |
E82Q | 90% relative enzyme activity | Saccharomyces cerevisiae |
E82Q | relative activity: 90% | Saccharomyces cerevisiae |
E98L | relative activity: 100% | Saccharomyces cerevisiae |
F178L | 34% relative enzyme activity | Saccharomyces cerevisiae |
F178L | relative activity: 34% | Saccharomyces cerevisiae |
F183L | 100% relative enzyme activity | Saccharomyces cerevisiae |
F188L | 2% relative enzyme activity | Saccharomyces cerevisiae |
F188L | relative activity: 2% | Saccharomyces cerevisiae |
F220L | 29% relative enzyme activity | Saccharomyces cerevisiae |
F220L | relative activity: 29% | Saccharomyces cerevisiae |
F269L | no activity | Saccharomyces cerevisiae |
F269L | no enzyme activity | Saccharomyces cerevisiae |
F357L | 100% relative enzyme activity | Saccharomyces cerevisiae |
F357L | relative activity: 100% | Saccharomyces cerevisiae |
F89L | 100% relative enzyme activity | Saccharomyces cerevisiae |
F89L | relative activity: 100% | Saccharomyces cerevisiae |
F91L | 15% relative enzyme activity | Saccharomyces cerevisiae |
F91L | relative activity: 15% | Saccharomyces cerevisiae |
G127L | 1% relative enzyme activity | Saccharomyces cerevisiae |
G127L | relative activity: 1% | Saccharomyces cerevisiae |
G129L | no activity | Saccharomyces cerevisiae |
G129L | no enzyme activity | Saccharomyces cerevisiae |
G131L | 5% relative enzyme activity | Saccharomyces cerevisiae |
G131L | relative activity: 5% | Saccharomyces cerevisiae |
G132L | 50% relative enzyme activity | Saccharomyces cerevisiae |
G132L | relative activity: 50% | Saccharomyces cerevisiae |
G217L | 100% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols | Saccharomyces cerevisiae |
G217L | relative activity: 100% | Saccharomyces cerevisiae |
G218L | 100% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols | Saccharomyces cerevisiae |
G218L | relative activity: 100% | Saccharomyces cerevisiae |
G347L | 5% relative enzyme activity | Saccharomyces cerevisiae |
G347L | relative activity: 5% | Saccharomyces cerevisiae |
G351L | 90% relative enzyme activity | Saccharomyces cerevisiae |
G351L | relative activity: 90% | Saccharomyces cerevisiae |
G352L | 3% relative enzyme activity | Saccharomyces cerevisiae |
G352L | relative activity: 3% | Saccharomyces cerevisiae |
G84L | 14% relative enzyme activity | Saccharomyces cerevisiae |
G84L | relative activity: 14% | Saccharomyces cerevisiae |
G86L | 16% relative enzyme activity | Saccharomyces cerevisiae |
G86L | relative activity: 16% | Saccharomyces cerevisiae |
H107L | relative activity: 23% | Saccharomyces cerevisiae |
H199L | relative activity: 2% | Saccharomyces cerevisiae |
H199Q | 45% relative enzyme activity | Saccharomyces cerevisiae |
H199Q | relative activity: 45% | Saccharomyces cerevisiae |
H199R | 20% relative enzyme activity | Saccharomyces cerevisiae |
H199R | relative activity: 20% | Saccharomyces cerevisiae |
H238L | relative activity: 85% | Saccharomyces cerevisiae |
H90L | no activity | Saccharomyces cerevisiae |
H90Q | no activity | Saccharomyces cerevisiae |
H90Q | no enzyme activity | Saccharomyces cerevisiae |
H90R | no activity | Saccharomyces cerevisiae |
H90R | no enzyme activity | Saccharomyces cerevisiae |
I194L | 67% relative enzyme activity | Saccharomyces cerevisiae |
I194L | relative activity: 67% | Saccharomyces cerevisiae |
K215L | 100% relative enzyme activity | Saccharomyces cerevisiae |
K215L | relative activity: 100% | Saccharomyces cerevisiae |
K353L | 56% relative enzyme activity | Saccharomyces cerevisiae |
additional information | scanning mutagenesis experiments involving a leucine replacement of 52 amino acids in Erg6p followed by substitution of key residues with functionally or structurally similar amino acids indicate that 5 new residues at positions Y192, G217, G218, T219 and Y223 can switch the course of C1-transfer activity to include plant-like C2-transfer activity. The data support a model in which several conserved and non-conserved amino acids located in distinct regions of the Saccharomyces cerevisiae Erg6p regulate the course of the C-methylation reaction toward product differences | Saccharomyces cerevisiae |
P133L | no activity | Saccharomyces cerevisiae |
P133L | no enzyme activity | Saccharomyces cerevisiae |
P201L | 58% relative enzyme activity | Saccharomyces cerevisiae |
P201L | relative activity: 58% | Saccharomyces cerevisiae |
P216L | 65% relative enzyme activity | Saccharomyces cerevisiae |
P216L | relative activity: 65% | Saccharomyces cerevisiae |
S354L | 100% relative enzyme activity | Saccharomyces cerevisiae |
S354L | relative activity: 100% | Saccharomyces cerevisiae |
S87E | 100% relative enzyme activity | Saccharomyces cerevisiae |
S87L | 100% relative enzyme activity | Saccharomyces cerevisiae |
S87L | relative activity: 100% | Saccharomyces cerevisiae |
S87Q | 100% relative enzyme activity | Saccharomyces cerevisiae |
S88L | 61% relative enzyme activity | Saccharomyces cerevisiae |
S88L | relative activity: 61% | Saccharomyces cerevisiae |
S88N | 17% relative enzyme activity | Saccharomyces cerevisiae |
S88N | relative activity: 17% | Saccharomyces cerevisiae |
S97E | relative activity: 100% | Saccharomyces cerevisiae |
S97Q | relative activity: 100% | Saccharomyces cerevisiae |
T197L | 93% relative enzyme activity | Saccharomyces cerevisiae |
T197L | relative activity: 93% | Saccharomyces cerevisiae |
T219L | 32% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols | Saccharomyces cerevisiae |
V126L | 1% relative enzyme activity | Saccharomyces cerevisiae |
V126L | relative activity: 1% | Saccharomyces cerevisiae |
V130L | 100% relative enzyme activity | Saccharomyces cerevisiae |
V130L | relative activity: 100% | Saccharomyces cerevisiae |
V222L | 27% relative enzyme activity | Saccharomyces cerevisiae |
V349L | 47% relative enzyme activity | Saccharomyces cerevisiae |
V349L | relative activity: 47% | Saccharomyces cerevisiae |
W225F | 100% relative enzyme activity | Saccharomyces cerevisiae |
W225F | relative activity: 60% | Saccharomyces cerevisiae |
W225L | 100% relative enzyme activity | Saccharomyces cerevisiae |
W225L | relative activity: 100% | Saccharomyces cerevisiae |
W286F | 100% relative enzyme activity | Saccharomyces cerevisiae |
W286F | relative activity: 51% | Saccharomyces cerevisiae |
W286L | 2% relative enzyme activity | Saccharomyces cerevisiae |
W286L | relative activity: 2% | Saccharomyces cerevisiae |
W85F | 100% relative enzyme activity | Saccharomyces cerevisiae |
W85F | relative activity: 55% | Saccharomyces cerevisiae |
W85L | 40% relative enzyme activity | Saccharomyces cerevisiae |
W85L | relative activity: 40% | Saccharomyces cerevisiae |
Y153F | 100% relative enzyme activity | Saccharomyces cerevisiae |
Y153F | relative activity: 41% | Saccharomyces cerevisiae |
Y153L | no enzyme activity | Saccharomyces cerevisiae |
Y153L | relative activity: 100% | Saccharomyces cerevisiae |
Y192F | 100% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols | Saccharomyces cerevisiae |
Y192L | 95% relative enzyme activity | Saccharomyces cerevisiae |
Y207F | 100% relative enzyme activity | Saccharomyces cerevisiae |
Y207F | relative activity: 100% | Saccharomyces cerevisiae |
Y207L | 3% relative enzyme activity | Saccharomyces cerevisiae |
Y207L | relative activity: 3% | Saccharomyces cerevisiae |
Y223F | 100% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols | Saccharomyces cerevisiae |
Y223F | relative activity: 100% | Saccharomyces cerevisiae |
Y223L | 100% relative enzyme activity | Saccharomyces cerevisiae |
Y223L | relative activity: 100% | Saccharomyces cerevisiae |
Y287L | 20% relative enzyme activity | Saccharomyces cerevisiae |
Y287L | relative activity: 20% | Saccharomyces cerevisiae |
Y74F | 100% relative enzyme activity | Saccharomyces cerevisiae |
Y74F | relative activity: 100% | Saccharomyces cerevisiae |
Y74L | 100% relative enzyme activity | Saccharomyces cerevisiae |
Y74L | relative activity: 100% | Saccharomyces cerevisiae |
Y81F | relative activity: 100% | Saccharomyces cerevisiae |
Y81L | 90% relative enzyme activity | Saccharomyces cerevisiae |
Y81L | relative activity: 90% | Saccharomyces cerevisiae |
Y83F | 95% relative enzyme activity | Saccharomyces cerevisiae |
Y83F | relative activity: 95% | Saccharomyces cerevisiae |
Y83L | 90% relative enzyme activity | Saccharomyces cerevisiae |
Y83L | relative activity: 90% | Saccharomyces cerevisiae |
Y83W | 100% relative enzyme activity | Saccharomyces cerevisiae |
Y83W | relative activity: 100% | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | P25087 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Saccharomyces cerevisiae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
prediction of secondary structure, eight residues at positions 79, 81, 82, 192, 217, 218, 219 and 223 critical for catalysis, scanning mutagenesis experiments involving a leucine replacement of 52 amino acids, substitution of key residues with functionally or structurally similar amino acids, 5 new residues at positions Y192, G217, G218, T219 and Y223 can switch the course of C1-transfer activity to include plant-like C2-transfer activity, model supported in which several conserved and non-conserved amino acids located in distinct regions regulate course of the C-methylation reaction toward product differences | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | formation of the ergostane (C1-transfer activity) and stigmastane (C2-transfer activity) sterol side chains, stepwise mutagenesis of amino acids of Erg6p, identification of five residues within the putative active site that contribute directly to C-methylation pathways operated by fungal and plant SMT proteins | Saccharomyces cerevisiae | ? | - |
? | |
S-adenosyl-L-methionine + zymosterol | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + fecosterol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Erg6p | - |
Saccharomyces cerevisiae |
SMT protein | - |
Saccharomyces cerevisiae |
SMT1 | - |
Saccharomyces cerevisiae |
sterol C24-methyltransferase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |