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Literature summary for 2.1.1.41 extracted from
- Enzyme redesign and interactions of substrate analogues with sterol methyltransferase to understand phytosterol diversity, reaction mechanism and the nature of the active site (2005), Biochem. Soc. Trans., 33, 1189-1196.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | active site of the yeast SMT has the necessary amino acids to generate products common to SMT catalysis of plants and protozoa, minor perturbations in the active site topography brought about by mutagenesis are sufficient to recognize new substrates | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E82L | produces plant-like substrate-product profiles | Saccharomyces cerevisiae |
| Y81F | produces plant-like substrate-product profiles | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 24(R,S),25-epiminozymosterol | reversible inhibitor, disrupts ergosterol homoeostasis by interrupting methyl addition to C-24 thereby impairing growth | Saccharomyces cerevisiae |  |
| 25-azalanosterol | - |
Saccharomyces cerevisiae | |
| 26,27-dehydrozymosterol | irreversible inhibitor that binds covalently to the SMT, affinitylabels the active site to convert to a product characterized as delta23(24)-sterol | Saccharomyces cerevisiae | |
| ergosterol | reversible inhibitor | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
| Fusarium sp. | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Trypanosoma brucei | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lanosterol + S-adenosyl-L-methionine | - |
Fusarium sp. | 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine | - |
? | |
| additional information | minimum requirements for productive binding and formation of catalytically active enzyme-substrate complex must be a substrate that possesses a 3beta-hydroxy group, a planar nucleus, an intact side chain that retains the length of the native substrate zymosterol, a 20-R configuration and a delta24-double bond | Saccharomyces cerevisiae | ? | - |
? | |
| zymosterol + S-adenosyl-L-methionine | - |
Trypanosoma brucei | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
| zymosterol + S-adenosyl-L-methionine | a bisubstrate reaction that proceeds by a sequential and noncovalent reaction whereby beta-face methyl addition to the DELTA24-bond and deprotonation of C-28 proceed to give rise to a nucleophilic rearrangement in which H-24 migrates to C-25 on the reface of the substrate double bond in concert with the initial ionization to afford the bound fecosterol | Saccharomyces cerevisiae | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SMT1 | - |
Saccharomyces cerevisiae |
| SMT1 | - |
Trypanosoma brucei |
| SMT1 | - |
Candida albicans |
| SMT1 | - |
Fusarium sp. |
| sterol methyltransferase | - |
Saccharomyces cerevisiae |
| sterol methyltransferase | - |
Trypanosoma brucei |
| sterol methyltransferase | - |
Candida albicans |
| sterol methyltransferase | - |
Fusarium sp. |
| STM | - |
Saccharomyces cerevisiae |
| STM | - |
Trypanosoma brucei |
| STM | - |
Candida albicans |
| STM | - |
Fusarium sp. |
| zymosterol 24-methyltransferase | - |
Saccharomyces cerevisiae |
| zymosterol 24-methyltransferase | - |
Trypanosoma brucei |
| zymosterol 24-methyltransferase | - |
Candida albicans |
| zymosterol 24-methyltransferase | - |
Fusarium sp. |
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