Literature summary for 2.1.1.41 extracted from

Nes, W.D.; Jayasimha, P.; Zhou, W.; Kanagasabai, R.; Jin, C.; Jaradat, T.T.; Shaw, R.W.; Bujnicki, J.M.
Sterol methyltransferase: functional analysis of highly conserved residues by site-directed mutagenesis (2004), Biochemistry, 43, 569-576.

Search for more literature for 2.1.1.41

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, wild-type and mutant enzymes	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
D125L	inactive mutant	Saccharomyces cerevisiae
D152L	inactive mutant	Saccharomyces cerevisiae
D189L	the ratio of turnover-number to KM-value for zymosterol is 1.4fold lower than the wild-type ratio	Saccharomyces cerevisiae
D276L	inactive mutant	Saccharomyces cerevisiae
E108L	the ratio of turnover-number to KM-value for zymosterol is 11fold lower than the wild-type ratio	Saccharomyces cerevisiae
E195L	inactive mutant	Saccharomyces cerevisiae
E209L	the ratio of turnover-number to KM-value for zymosterol is 2.4fold lower than the wild-type ratio	Saccharomyces cerevisiae
E246L	the ratio of turnover-number to KM-value for zymosterol is 13.5fold lower than the wild-type ratio	Saccharomyces cerevisiae
H107L	the ratio of turnover-number to KM-value for zymosterol is 2.4fold lower than the wild-type ratio	Saccharomyces cerevisiae
H199L	the ratio of turnover-number to KM-value for zymosterol is 162fold lower than the wild-type ratio	Saccharomyces cerevisiae
H224L	the ratio of turnover-number to KM-value for zymosterol is 1.4fold lower than the wild-type ratio	Saccharomyces cerevisiae
H238L	the ratio of turnover-number to KM-value for zymosterol is 1.6fold lower than the wild-type ratio	Saccharomyces cerevisiae
H90L	inactive mutant	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
25-azalanosterol	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.011	-	Zymosterol	pH 7.5, 32°C, mutant enzyme H107L	Saccharomyces cerevisiae
0.017	-	Zymosterol	pH 7.5, 32°C, mutant enzyme D189L	Saccharomyces cerevisiae
0.017	-	Zymosterol	pH 7.5, 32°C, mutant enzyme E224L	Saccharomyces cerevisiae
0.017	-	Zymosterol	pH 7.5, 32°C, wild-type enzyme	Saccharomyces cerevisiae
0.02	-	Zymosterol	pH 7.5, 32°C, mutant enzyme H238L	Saccharomyces cerevisiae
0.024	-	Zymosterol	pH 7.5, 32°C, mutant enzyme H199L	Saccharomyces cerevisiae
0.034	-	Zymosterol	pH 7.5, 32°C, mutant enzyme E108L	Saccharomyces cerevisiae
0.034	-	Zymosterol	pH 7.5, 32°C, mutant enzyme E209L	Saccharomyces cerevisiae
0.042	-	Zymosterol	pH 7.5, 32°C, mutant enzyme E246L	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and mutant enzymes	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + zymosterol	the active center is composed of a set of acidic amino acids, Asp125, Asp152, Glu195 and Asp276, which contribute to initial binding of sterol and S-adenosyl-L-methionine. His90 functions subsequently in the reaction process to promote product formation	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + fecosterol	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0001	-	Zymosterol	pH 7.5, 32°C, mutant enzyme H199L	Saccharomyces cerevisiae
0.002	-	Zymosterol	pH 7.5, 32°C, mutant enzyme E108L	Saccharomyces cerevisiae
0.002	-	Zymosterol	pH 7.5, 32°C, mutant enzyme E246L	Saccharomyces cerevisiae
0.003	-	Zymosterol	pH 7.5, 32°C, mutant enzyme H107L	Saccharomyces cerevisiae
0.008	-	Zymosterol	pH 7.5, 32°C, mutant enzyme D189L	Saccharomyces cerevisiae
0.008	-	Zymosterol	pH 7.5, 32°C, mutant enzyme E224L	Saccharomyces cerevisiae
0.008	-	Zymosterol	pH 7.5, 32°C, mutant enzyme H238L	Saccharomyces cerevisiae
0.009	-	Zymosterol	pH 7.5, 32°C, mutant enzyme E209L	Saccharomyces cerevisiae
0.011	-	Zymosterol	pH 7.5, 32°C, wild-type enzyme	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)